Uc.416?+?A promotes epithelial-to-mesenchymal transition through miR-153 in renal cell carcinoma.

ABSTRACT:

Background

The transcribed ultraconserved regions (T-UCRs) are a novel class of non-coding RNAs that are absolutely conserved across species and are involved in carcinogenesis in some cancers. However, the expression and biological role of T-UCRs in renal cell carcinoma (RCC) remain poorly understood. This study aimed to examine the expression and functional role of Uc.416?+?A and analyze the association between Uc.416?+?A and epithelial-to-mesenchymal transition in RCC.

Methods

Expression of Uc.416?+?A in 35 RCC tissues, corresponding normal kidney tissues and 13 types of normal tissue samples was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We performed a cell growth and migration assay in RCC cell line 786-O transfected with negative control and siRNA for Uc.416?+?A. We evaluated the relation between Uc.416?+?A and miR-153, which has a complimentary site of Uc.416?+?A.

Results

qRT-PCR analysis revealed that the expression of Uc.416?+?A was higher in RCC tissues than that in corresponding normal kidney tissues. Inhibition of Uc.416?+?A reduced cell growth and cell migration activity. There was an inverse correlation between Uc.416?+?A and miR-153. Western blot analysis showed Uc.416?+?A modulated E-cadherin, vimentin and snail. The expression of Uc.416?+?A was positively associated with the expression of SNAI1, VIM and inversely associated with the expression of CDH1.

Conclusions

The expression of Uc.416?+?A was upregulated in RCC and especially in RCC tissues with sarcomatoid change. Uc.416?+?A promoted epithelial-to-mesenchymal transition through miR-153. These results suggest that Uc.416?+?A may be a promising therapeutic target.

SUBMITTER: Sekino Y 

PROVIDER: S-EPMC6172711 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature