Dataset Information

Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence.

ABSTRACT: OBJECTIVE:The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil. METHOD:One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus primers MY09/MY11 and GP5+/GP6+, followed by nested multiplex polymerase chain reaction with type-specific primers for the E6/E7 consensus region. RESULTS:Human papillomavirus DNA was detected in 51 (49.5%) breast carcinoma samples and 15 (15.8%) normal breast samples (p<0.0001). Human papillomavirus genotypes 6 and 11 were identified in 15.2% of all samples. CONCLUSIONS:The high frequency of human papillomavirus infection in breast cancer samples indicates a potential role of this virus in breast carcinogenesis in the studied participants.

SUBMITTER: Cavalcante JR

PROVIDER: S-EPMC6172977 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence.

Cavalcante José Roosevelt JR Pinheiro Luiz Gonzaga Porto LGP Almeida Paulo Roberto Carvalho de PRC Ferreira Márcia Valéria Pitombeira MVP Cruz Gizele Almada GA Campelo Thales Alves TA Silva Caroliny Soares CS Lima Luana Nepomuceno Gondim Costa LNGC Oliveira Bruno Masato Kitagawa de BMK Lima Lara Mulato LM Feitosa Laura Magda Costa LMC Pinheiro Agostinho Câmara AC Frota Cristiane Cunha CC

Clinics (Sao Paulo, Brazil) 20181018

<h4>Objective</h4>The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil.<h4>Method</h4>One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus pri ...[more]

PMID: 30365827

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Project description:BACKGROUND:Genetic factors may influence the susceptibility to high-risk (hr) human papillomavirus (HPV) infection and persistence. We conducted the first genome-wide association study (GWAS) to identify variants associated with cervical hrHPV infection and persistence. METHODS:Participants were 517 Nigerian women evaluated at baseline and 6?months follow-up visits for HPV. HPV was characterized using SPF10/LiPA25. hrHPV infection was positive if at least one carcinogenic HPV genotype was detected in a sample provided at the baseline visit and persistent if at least one carcinogenic HPV genotype was detected in each of the samples provided at the baseline and follow-up visits. Genotyping was done using the Illumina Multi-Ethnic Genotyping Array (MEGA) and imputation was done using the African Genome Resources Haplotype Reference Panel. Association analysis was done for hrHPV infection (125 cases/392 controls) and for persistent hrHPV infection (51 cases/355 controls) under additive genetic models adjusted for age, HIV status and the first principal component (PC) of the genotypes. RESULTS:The mean (±SD) age of the study participants was 38 (±8) years, 48% were HIV negative, 24% were hrHPV positive and 10% had persistent hrHPV infections. No single variant reached genome-wide significance (p?<?5 X 10-?8). The top three variants associated with hrHPV infections were intronic variants clustered in KLF12 (all OR: 7.06, p?=?1.43?×?10-?6). The top variants associated with cervical hrHPV persistence were in DAP (OR: 6.86, p?=?7.15?×?10-?8), NR5A2 (OR: 3.65, p?=?2.03?×?10-?7) and MIR365-2 (OR: 7.71, p?=?2.63?×?10-?7) gene regions. CONCLUSIONS:This exploratory GWAS yielded suggestive candidate risk loci for cervical hrHPV infection and persistence. The identified loci have biological annotation and functional data supporting their role in hrHPV infection and persistence. Given our limited sample size, larger discovery and replication studies are warranted to further characterize the reported associations.

Dataset Information

Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence.

Publications

Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence.

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