Ontology highlight
ABSTRACT:
SUBMITTER: Lynch RW
PROVIDER: S-EPMC6175317 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Lynch Ruairi W RW Hawley Catherine A CA Pellicoro Antonella A Bain Calum C CC Iredale John P JP Jenkins Stephen J SJ
Journal of leukocyte biology 20180401 3
Multicolor flow cytometry and cell sorting are powerful immunologic tools for the study of hepatic mϕ, yet there is no consensus on the optimal method to prepare liver homogenates for these analyses. Using a combination of mϕ and endothelial cell reporter mice, flow cytometry, and confocal imaging, we have shown that conventional flow-cytometric strategies for identification of Kupffer cells (KCs) leads to inclusion of a significant proportion of CD31<sup>hi</sup> endothelial cells. These cells ...[more]