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Changes in the genetic requirements for microbial interactions with increasing community complexity.

ABSTRACT: Microbial community structure and function rely on complex interactions whose underlying molecular mechanisms are poorly understood. To investigate these interactions in a simple microbiome, we introduced E. coli into an experimental community based on a cheese rind and identified the differences in E. coli's genetic requirements for growth in interactive and non-interactive contexts using Random Barcode Transposon Sequencing (RB-TnSeq) and RNASeq. Genetic requirements varied among pairwise growth conditions and between pairwise and community conditions. Our analysis points to mechanisms by which growth conditions change as a result of increasing community complexity and suggests that growth within a community relies on a combination of pairwise and higher-order interactions. Our work provides a framework for using the model organism E. coli as a readout to investigate microbial interactions regardless of the genetic tractability of members of the studied ecosystem.

SUBMITTER: Morin M

PROVIDER: S-EPMC6175579 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Changes in the genetic requirements for microbial interactions with increasing community complexity.

Morin Manon M Pierce Emily C EC Dutton Rachel J RJ

eLife 20180913

Microbial community structure and function rely on complex interactions whose underlying molecular mechanisms are poorly understood. To investigate these interactions in a simple microbiome, we introduced <i>E. coli</i> into an experimental community based on a cheese rind and identified the differences in <i>E. coli'</i>s genetic requirements for growth in interactive and non-interactive contexts using Random Barcode Transposon Sequencing (RB-TnSeq) and RNASeq. Genetic requirements varied among ...[more]

PMID: 30211673

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