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Structural mechanism of Myb-MuvB assembly.

ABSTRACT: The MuvB transcriptional regulatory complex, which controls cell-cycle-dependent gene expression, cooperates with B-Myb to activate genes required for the G2 and M phases of the cell cycle. We have identified the domain in B-Myb that is essential for the assembly of the Myb-MuvB (MMB) complex. We determined a crystal structure that reveals how this B-Myb domain binds MuvB through the adaptor protein LIN52 and the scaffold protein LIN9. The structure and biochemical analysis provide an understanding of how oncogenic B-Myb is recruited to regulate genes required for cell-cycle progression, and the MMB interface presents a potential therapeutic target to inhibit cancer cell proliferation.

SUBMITTER: Guiley KZ

PROVIDER: S-EPMC6176624 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Structural mechanism of Myb-MuvB assembly.

Guiley Keelan Z KZ Iness Audra N AN Saini Siddharth S Tripathi Sarvind S Lipsick Joseph S JS Litovchick Larisa L Rubin Seth M SM

Proceedings of the National Academy of Sciences of the United States of America 20180917 40

The MuvB transcriptional regulatory complex, which controls cell-cycle-dependent gene expression, cooperates with B-Myb to activate genes required for the G2 and M phases of the cell cycle. We have identified the domain in B-Myb that is essential for the assembly of the Myb-MuvB (MMB) complex. We determined a crystal structure that reveals how this B-Myb domain binds MuvB through the adaptor protein LIN52 and the scaffold protein LIN9. The structure and biochemical analysis provide an understand ...[more]

PMID: 30224471

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