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Induction of human somatostatin and parvalbumin neurons by expressing a single transcription factor LIM homeobox 6.


ABSTRACT: Human GABAergic interneurons (GIN) are implicated in normal brain function and in numerous mental disorders. However, the generation of functional human GIN subtypes from human pluripotent stem cells (hPSCs) has not been established. By expressing LHX6, a transcriptional factor that is critical for GIN development, we induced hPSCs to form GINs, including somatostatin (SST, 29%) and parvalbumin (PV, 21%) neurons. Our RNAseq results also confirmed the alteration of GIN identity with the overexpression of LHX6. Five months after transplantation into the mouse brain, the human GABA precursors generated increased population of SST and PV neurons by overexpressing LHX6. Importantly, the grafted human GINs exhibited functional electrophysiological properties and even fast-spiking-like action potentials. Thus, expression of the single transcription factor LHX6 under our GIN differentiation condition is sufficient to robustly induce human PV and SST subtypes.

SUBMITTER: Yuan F 

PROVIDER: S-EPMC6181563 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Induction of human somatostatin and parvalbumin neurons by expressing a single transcription factor LIM homeobox 6.

Yuan Fang F   Chen Xin X   Fang Kai-Heng KH   Wang Yuanyuan Y   Lin Mingyan M   Xu Shi-Bo SB   Huo Hai-Qin HQ   Xu Min M   Ma Lixiang L   Chen Yuejun Y   He Shuijin S   Liu Yan Y  

eLife 20180925


Human GABAergic interneurons (GIN) are implicated in normal brain function and in numerous mental disorders. However, the generation of functional human GIN subtypes from human pluripotent stem cells (hPSCs) has not been established. By expressing LHX6, a transcriptional factor that is critical for GIN development, we induced hPSCs to form GINs, including somatostatin (SST, 29%) and parvalbumin (PV, 21%) neurons. Our RNAseq results also confirmed the alteration of GIN identity with the overexpre  ...[more]

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