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The antioxidant activities, cytotoxic properties, and identification of water-soluble compounds of Ficus deltoidea leaves.

ABSTRACT: Leaves from three varieties of Ficus deltoidea, colloquially termed small- (FDS), medium- (FDM), and big-type leaf (FDB), were subjected to water extraction. The crude extracts were fractionated using water (WF) and ethyl acetate (EAF). The phenolic and flavonoid content, antioxidant activity, and cytotoxicity of the fractions were investigated. The EAF had the highest phenolic and flavonoid content compared to the other FDS fractions. Conversely, the FDM crude extract had the highest phenolic and flavonoid content compared to the other FDM samples. Antioxidant activity was highest in the FDB crude extract. Ultra-high-performance liquid chromatography showed that two compounds, vitexin and coumaric acid, were present in the FDB crude extract. Additionally, the F. deltoidea leaves caused no signs of toxicity in a normal liver cell line. Our findings show that F. deltoidea varieties have excellent antioxidant activity with no cytotoxic effects on normal liver cells.

SUBMITTER: Abrahim NN

PROVIDER: S-EPMC6186405 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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The antioxidant activities, cytotoxic properties, and identification of water-soluble compounds of <i>Ficus deltoidea</i> leaves.

Abrahim Noor Nazirahanie NN Abdul-Rahman Puteri Shafinaz PS Aminudin Norhaniza N

PeerJ 20181011

Leaves from three varieties of <i>Ficus deltoidea</i>, colloquially termed small- (FDS), medium- (FDM), and big-type leaf (FDB), were subjected to water extraction. The crude extracts were fractionated using water (WF) and ethyl acetate (EAF). The phenolic and flavonoid content, antioxidant activity, and cytotoxicity of the fractions were investigated. The EAF had the highest phenolic and flavonoid content compared to the other FDS fractions. Conversely, the FDM crude extract had the highest phe ...[more]

PMID: 30324012

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Project description:The medicinal orchid Dendrobium moniliforme contains water-soluble polysaccharides, phenanthrenes, bibenzyl derivatives, and polyphenol compounds. This study explored the antioxidant and cytotoxic activities of D. moniliforme extracts and detected their bioactive compounds.Plant material was collected from the Daman of Makawanpur district in central Nepal. Plant extracts were prepared from stems using hexane, chloroform, acetone, ethanol and methanol. The total polyphenol content (TPC) in each extract was determined using Folin-Ciocalteu's reagent and the total flavonoid content (TFC) in each extract was determined using the aluminium chloride method. The in vitro antioxidant and cytotoxic activities of each extract were determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays respectively. Gas chromatography and mass spectrometry (GC-MS) analysis was used to detect bioactive compounds.TPC content was highest (116.65 ?g GAE/mg of extract) in D. moniliforme chloroform extract (DMC) and TFC content was highest (116.67 ?g QE/mg of extract) in D. moniliforme acetone extract (DMA). D. moniliforme hexane extract (DMH) extract showed the highest percentage of DPPH radical scavenging activity (94.48%), followed closely by D. moniliforme ethanol extract (DME) (94.45%), DMA (93.71%) and DMC (94.35%) at 800 ?g/ml concentration. The antioxidant capacities of DMC, DMA, DMH and DME, which were measured in IC50 values, were much lower 42.39 ?g/ml, 49.56 ?g/ml, 52.68 ?g/ml, and 58.77 ?g/ml respectively than the IC50 of D. moniliforme methanol extract (DMM) (223.15 ?g/ml). DMM at the concentration of 800 ?g/ml most inhibited the growth of HeLa cells (78.68%) and DME at the same concentration most inhibited the growth of U251 cells (51.95%). The cytotoxic capacity (IC50) of DMM against HeLa cells was 155.80 ?g/ml of extract and that of DME against the U251 cells was 772.50 ?g/ml of extract. A number of bioactive compounds were detected in both DME and DMM.The fact that plant extract of D. moniliforme has a number of bioactive compounds which showed antioxidant and cytotoxic activities suggests the potential pharmacological importance of this plant.

Dataset Information

The antioxidant activities, cytotoxic properties, and identification of water-soluble compounds of Ficus deltoidea leaves.

Publications

The antioxidant activities, cytotoxic properties, and identification of water-soluble compounds of <i>Ficus deltoidea</i> leaves.

Similar Datasets

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