Dataset Information

Production of bioactive ginsenoside Rg3(S) and compound K using recombinant Lactococcus lactis.

ABSTRACT:

Background

Ginsenoside Rg3(S) and compound K (C-K) are pharmacologically active components of ginseng that promote human health and improve quality of life. The aim of this study was to produce Rg3(S) and C-K from ginseng extract using recombinant Lactococcus lactis.

Methods

L. lactis subsp. cremoris NZ9000 (L. lactis NZ9000), which harbors ?-glucosidase genes (BglPm and BglBX10) from Paenibacillus mucilaginosus and Flavobacterium johnsoniae, respectively, was reacted with ginseng extract (protopanaxadiol-type ginsenoside mixture).

Results

Crude enzyme activity of BglBX10 values comprised 0.001 unit/mL and 0.003 unit/mL in uninduced and induced preparations, respectively. When whole cells of L. lactis harboring pNZBglBX10 were treated with ginseng extract, after permeabilization of cells by xylene, Rb1 and Rd were converted into Rg3(S) with a conversion yield of 61%. C-K was also produced by sequential reactions of the permeabilized cells harboring each pNZBgl and pNZBglBX10, resulting in a 70% maximum conversion yield.

Conclusion

This study demonstrates that the lactic acid bacteria having specific ?-glucosidase activity can be used to enhance the health benefits of Panax ginseng in either fermented foods or bioconversion processes.

SUBMITTER: Li L

PROVIDER: S-EPMC6187048 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Publications

Production of bioactive ginsenoside Rg3(S) and compound K using recombinant <i>Lactococcus lactis</i>.

Li Ling L Lee Soo Jin SJ Yuan Qiu Ping QP Im Wan Taek WT Kim Sun Chang SC Han Nam Soo NS

Journal of ginseng research 20170421 4

<h4>Background</h4>Ginsenoside Rg3(S) and compound K (C-K) are pharmacologically active components of ginseng that promote human health and improve quality of life. The aim of this study was to produce Rg3(S) and C-K from ginseng extract using recombinant <i>Lactococcus lactis</i>.<h4>Methods</h4><i>L. lactis</i> subsp. <i>cremoris</i> NZ9000 (<i>L. lactis</i> NZ9000), which harbors β-glucosidase genes (<i>BglPm</i> and <i>BglBX10</i>) from <i>Paenibacillus mucilaginosus</i> and <i>Flavobacteriu ...[more]

PMID: 30337801

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