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A Horizontal Magnetic Tweezers and Its Use for Studying Single DNA Molecules.

ABSTRACT: We report the development of a magnetic tweezers that can be used to micromanipulate single DNA molecules by applying picoNewton (pN)-scale forces in the horizontal plane. The resulting force?extension data from our experiments show high-resolution detection of changes in the DNA tether's extension: ~0.5 pN in the force and <10 nm change in extension. We calibrate our instrument using multiple orthogonal techniques including the well-characterized DNA overstretching transition. We also quantify the repeatability of force and extension measurements, and present data on the behavior of the overstretching transition under varying salt conditions. The design and experimental protocols are described in detail, which should enable straightforward reproduction of the tweezers.

SUBMITTER: Fabian R

PROVIDER: S-EPMC6187538 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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A Horizontal Magnetic Tweezers and Its Use for Studying Single DNA Molecules.

Fabian Roberto R Tyson Christopher C Tuma Pamela L PL Pegg Ian I Sarkar Abhijit A

Micromachines 20180417 4

We report the development of a magnetic tweezers that can be used to micromanipulate single DNA molecules by applying picoNewton (pN)-scale forces in the horizontal plane. The resulting force⁻extension data from our experiments show high-resolution detection of changes in the DNA tether's extension: ~0.5 pN in the force and <10 nm change in extension. We calibrate our instrument using multiple orthogonal techniques including the well-characterized DNA overstretching transition. We also quantify ...[more]

PMID: 30424121

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