Dataset Information

Formation of Branched and Chained Alginate Microfibers Using Theta-Glass Capillaries.

ABSTRACT: This study proposes a microfluidic spinning method to form alginate microfibers with branched and chained structures by controlling two streams of a sodium alginate solution extruded from a theta-glass capillary (a double-compartmented glass capillary). The two streams have three flow regimes: (i) a combined flow regime (single-threaded stream), (ii) a separated flow regime (double-threaded stream), and (iii) a chained flow regime (stream of repeating single- and double-threaded streams). The flow rate of the sodium alginate solution and the tip diameter of the theta-glass capillary are the two parameters which decide the flow regime. By controlling the two parameters, we form branched (a Y-shaped structure composed of thick parent fiber and permanently divided two thin fibers) and chained (a repeating structure of single- and double-threaded fibers with constant frequency) alginate microfibers with various dimensions. Furthermore, we demonstrate the applicability of the alginate microfibers as sacrificial templates for the formation of chain-shaped microchannels with two inlets. Such microchannels could mimic the structure of blood vessels and are applicable for the research fields of fluidics including hemodynamics.

SUBMITTER: Nishimura K

PROVIDER: S-EPMC6187737 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Formation of Branched and Chained Alginate Microfibers Using Theta-Glass Capillaries.

Nishimura Keigo K Morimoto Yuya Y Mori Nobuhito N Takeuchi Shoji S

Micromachines 20180617 6

This study proposes a microfluidic spinning method to form alginate microfibers with branched and chained structures by controlling two streams of a sodium alginate solution extruded from a theta-glass capillary (a double-compartmented glass capillary). The two streams have three flow regimes: (i) a combined flow regime (single-threaded stream), (ii) a separated flow regime (double-threaded stream), and (iii) a chained flow regime (stream of repeating single- and double-threaded streams). The fl ...[more]

PMID: 30424236

Similar Datasets

Project description:Background 3D bioprinting cardiac patches for epicardial transplantation are a promising approach for myocardial regeneration. Challenges remain such as quantifying printability, determining the ideal moment to transplant, and promoting vascularisation within bioprinted patches. We aimed to evaluate 3D bioprinted cardiac patches for printability, durability in culture, cell viability, and endothelial cell structural self-organisation into networks. Methods We evaluated 3D-bioprinted double-layer patches using alginate/gelatine (AlgGel) hydrogels and three extrusion bioprinters (REGEMAT3D, INVIVO, BIO X). Bioink contained either neonatal mouse cardiac cell spheroids or free (not-in-spheroid) human coronary artery endothelial cells with fibroblasts, mixed with AlgGel. To test the effects on durability, some patches were bioprinted as a single layer only, cultured under minimal movement conditions or had added fibroblast-derived extracellular matrix hydrogel (AlloECM). Controls included acellular AlgGel and gelatin methacryloyl (GELMA) patches. Results Printability was similar across bioprinters. For AlgGel compared to GELMA: resolutions were similar (200–700 ?m line diameters), printing accuracy was 45 and 25%, respectively (AlgGel was 1.7x more accurate; p < 0.05), and shape fidelity was 92% (AlgGel) and 96% (GELMA); p = 0.36. For durability, AlgGel patch median survival in culture was 14 days (IQR:10–27) overall which was not significantly affected by bioprinting system or cellular content in patches. We identified three factors which reduced durability in culture: (1) bioprinting one layer depth patches (instead of two layers); (2) movement disturbance to patches in media; and (3) the addition of AlloECM to AlgGel. Cells were viable after bioprinting followed by 28 days in culture, and all BIO X-bioprinted mouse cardiac cell spheroid patches presented contractile activity starting between day 7 and 13 after bioprinting. At day 28, endothelial cells in hydrogel displayed organisation into endothelial network-like structures. Conclusion AlgGel-based 3D bioprinted heart patches permit cardiomyocyte contractility and endothelial cell structural self-organisation. After bioprinting, a period of 2 weeks maturation in culture prior to transplantation may be optimal, allowing for a degree of tissue maturation but before many patches start to lose integrity. We quantify AlgGel printability and present novel factors which reduce AlgGel patch durability (layer number, movement, and the addition of AlloECM) and factors which had minimal effect on durability (bioprinting system and cellular patch content). Graphical Abstract

Dataset Information

Formation of Branched and Chained Alginate Microfibers Using Theta-Glass Capillaries.

Publications

Formation of Branched and Chained Alginate Microfibers Using Theta-Glass Capillaries.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure