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The Wingate anaerobic test cannot be used for the evaluation of growth hormone secretion in children with short stature.

ABSTRACT: Purpose:To assess the growth hormone (GH) response to the Wingate anaerobic test (WAnT) among children with short stature and suspected GH deficiency. We hypothesized that the GH response to the WAnT would be similar to the GH response to a commonly used pharmacologic provocation test. Methods:Ten children (6 males and 4 females, age range 9.0-14.9 years) participated in the study. Each participant performed 2 tests: a standard all-out WAnT, cycling for 30?s against constant resistance, and a standardized pharmacologic test (clonidine or glucagon). Blood samples for GH were collected before and 10, 30, 45, and 60?min after the beginning of exercise. In addition, we collected pre- and post-exercise blood lactate levels. Results:There was a significant increase in GH levels after the WAnT, yet in 9 of 10 participants, this increase was below the threshold for GH sufficiency. Peak GH after the WAnT was significantly lower compared to the pharmacologic GH provocation tests (with 9 of 10 demonstrating GH-sufficient response). Conclusion:The traditional WAnT cannot be used as a GH provocation test. Further research is needed to develop anaerobic exercise protocols sufficient to promote GH secretion.

SUBMITTER: Dror N

PROVIDER: S-EPMC6189253 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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The Wingate anaerobic test cannot be used for the evaluation of growth hormone secretion in children with short stature.

Dror Nitzan N Oren Liat L Pantanowitz Michal M Eliakim Alon A Nemet Dan D

Journal of sport and health science 20160624 4

<h4>Purpose</h4>To assess the growth hormone (GH) response to the Wingate anaerobic test (WAnT) among children with short stature and suspected GH deficiency. We hypothesized that the GH response to the WAnT would be similar to the GH response to a commonly used pharmacologic provocation test.<h4>Methods</h4>Ten children (6 males and 4 females, age range 9.0-14.9 years) participated in the study. Each participant performed 2 tests: a standard all-out WAnT, cycling for 30 s against constant resis ...[more]

PMID: 30356652

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Project description:IntroductionThe growth hormone deficiency (GHD) diagnosis is controversial especially due to low specificity of growth hormone (GH) stimulation tests. It is therefore believed that children diagnosed with GHD form a heterogeneous group with growth disorder frequently independent on GH function. No study evaluating the complex etiology of growth failure in children with diagnosed GHD has been performed thus far.AimsTo discover genetic etiology of short stature in children with diagnosed GHD from families with short stature.MethodsFifty-two children diagnosed with primary GHD and vertically transmitted short stature (height SDS in the child and his/her shorter parent <-2 SD) were included to our study. The GHD diagnosis was based on growth data suggestive of GHD, absence of substantial disproportionality (sitting height to total height ratio <-2 SD or >+2 SD), IGF-1 levels <0 for age and sex specific SD and peak GH concentration <10 ug/L in two stimulation tests. All children were examined using next-generation sequencing methods, and the genetic variants were subsequently evaluated by American College of Medical Genetics standards and guidelines.ResultsThe age of children at enrollment into the study was 11 years (median, IQR 9-14 years), their height prior to GH treatment was -3.0 SD (-3.6 to -2.8 SD), IGF-1 concentration -1.4 SD (-2.0 to -1.1 SD), and maximal stimulated GH 6.3 ug/L (4.8-7.6 ug/L). No child had multiple pituitary hormone deficiency or a midbrain region pathology. Causative variant in a gene that affects growth was discovered in 15/52 (29%) children. Of them, only 2 (13%) had a genetic variant affecting GH secretion or function (GHSR and OTX2). Interestingly, in 10 (67%) children we discovered a primary growth plate disorder (ACAN, COL1A2, COL11A1, COL2A1, EXT2, FGFR3, NF1, NPR2, PTPN11 [2x]), in one (7%) a genetic variant impairing IGF-1 action (IGFALS) and in two (12%) a variant in miscellaneous genes (SALL4, MBTPS2).ConclusionsIn children with vertically transmitted short stature, genetic results frequently did not correspond with the clinical diagnosis of GH deficiency. These results underline the doubtful reliability of methods standardly used to diagnose GH deficiency.

Dataset Information

The Wingate anaerobic test cannot be used for the evaluation of growth hormone secretion in children with short stature.

Publications

The Wingate anaerobic test cannot be used for the evaluation of growth hormone secretion in children with short stature.

Similar Datasets

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