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Fabrication of a Cell Fixation Device for Robotic Cell Microinjection.

ABSTRACT: Automation of cell microinjection greatly reduces operational difficulty, but cell fixation remains a challenge. Here, we describe an innovative device that solves the fixation problem without single-cell operation. The microarray cylinder is designed with a polydimethylsiloxane (PDMS) material surface to control the contact force between cells and the material. Data show that when the injection velocity exceeds 1.5 mm/s, microinjection success rate is over 80%. The maximum value of the adhesion force between the PDMS plate and the cell is 0.0138 N, and the need can be met in practical use of the robotic microinjection.

SUBMITTER: Xie Y

PROVIDER: S-EPMC6190162 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

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Fabrication of a Cell Fixation Device for Robotic Cell Microinjection.

Xie Yu Y Zhou Yunlei Y Xi Wenming W Zeng Feng F Chen Songyue S

Micromachines 20160804 8

Automation of cell microinjection greatly reduces operational difficulty, but cell fixation remains a challenge. Here, we describe an innovative device that solves the fixation problem without single-cell operation. The microarray cylinder is designed with a polydimethylsiloxane (PDMS) material surface to control the contact force between cells and the material. Data show that when the injection velocity exceeds 1.5 mm/s, microinjection success rate is over 80%. The maximum value of the adhesion ...[more]

PMID: 30404304

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