Dataset Information

Genetic and population analyses of Vibrio parahaemolyticus isolates from three major coastal regions in China.

ABSTRACT: AIM:This study aims to evaluate the genetic and population structure of Vibrio parahaemolyticus in the major coastal regions of China. MATERIALS & METHODS:Multilocus sequence typing was performed. RESULTS:Insertion of large sequence into recA happened in nearly 30 strains, which were untypeable by multilocus sequence typing. A collection of 307 V. parahaemolyticus isolates were typed into 160 sequence types, including 117 novel ones. eBURST analysis revealed five clonal complexes, 11 doublets, and 108 singletons. The 160 sequence types formed two main lineages in the phylogenetic analysis. CONCLUSION:V. parahaemolyticus along the Chinese coastal regions exhibits high levels of genetic diversity and has undergone significant purifying selection and frequent recombination. A deeper understanding of V. parahaemolyticus genetic diversity could be obtained at the level of genome sequences.

SUBMITTER: Li P

PROVIDER: S-EPMC6190215 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Genetic and population analyses of Vibrio parahaemolyticus isolates from three major coastal regions in China.

Li Ping P Xin Wenwen W Kang Lin L Chen Zhongwen Z Guo Chenyi C Gao Shan S Yang Hao H Ji Bin B Yan Yong Y Wang Henghui H Zhou Dongsheng D Yang Wenhui W Wang Jinglin J

Future microbiology 20180921

<h4>Aim</h4>This study aims to evaluate the genetic and population structure of Vibrio parahaemolyticus in the major coastal regions of China.<h4>Materials & methods</h4>Multilocus sequence typing was performed.<h4>Results</h4>Insertion of large sequence into recA happened in nearly 30 strains, which were untypeable by multilocus sequence typing. A collection of 307 V. parahaemolyticus isolates were typed into 160 sequence types, including 117 novel ones. eBURST analysis revealed five clonal com ...[more]

PMID: 30238770

Similar Datasets

Project description:BackgroundVibrio parahaemolyticus is frequently isolated from environmental and seafood samples and associated with gastroenteritis outbreakes in American, European, Asian and African countries. To distinguish between different lineages of V. parahaemolyticus various genotyping techniques have been used, incl. multilocus sequence typing (MLST). Even though some studies have already applied MLST analysis to characterize V. parahaemolyticus strain sets, these studies have been restricted to specific geographical areas (e.g. U.S. coast, Thailand and Peru), have focused exclusively on pandemic or non-pandemic pathogenic isolates or have been based on a limited strain number.ResultsTo generate a global picture of V. parahaemolyticus genotype distribution, a collection of 130 environmental and seafood related V. parahaemolyticus isolates of different geographical origins (Sri Lanka, Ecuador, North Sea and Baltic Sea as well as German retail) was subjected to MLST analysis after modification of gyrB and recA PCRs. The V. parahaemolyticus population was composed of 82 unique Sequence Types (STs), of which 68 (82.9%) were new to the pubMLST database. After translating the in-frame nucleotide sequences into amino acid sequences, less diversity was detectable: a total of 31 different peptide Sequence Types (pSTs) with 19 (61.3%) new pSTs were generated from the analyzed isolates. Most STs did not show a global dissemination, but some were supra-regionally distributed and clusters of STs were dependent on geographical origin. On peptide level no general clustering of strains from specific geographical regions was observed, thereby the most common pSTs were found on all continents (Asia, South America and Europe) and rare pSTs were restricted to distinct countries or even geographical regions. One lineage of pSTs associated only with strains from North and Baltic Sea strains was identified.ConclusionsOur study reveals a high genetic diversity in the analyzed V. parahaemolyticus strain set as well as for geographical strain subsets, with a high proportion of newly discovered alleles and STs. Differences between the subsets were identified. Our data support the postulated population structure of V. parahaemolyticus which follows the 'epidemic' model of clonal expansion. Application of peptide based AA-MLST allowed the identification of reliable relationships between strains.

Dataset Information

Genetic and population analyses of Vibrio parahaemolyticus isolates from three major coastal regions in China.

Publications

Genetic and population analyses of Vibrio parahaemolyticus isolates from three major coastal regions in China.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets