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Label-Free Monitoring of Diffusion in Microfluidics.


ABSTRACT: Label-free, real-time detection of concentration gradients is demonstrated in a microfluidic H-filter, using an integrated photonic crystal slab sensor to monitor sample refractive index with spatial resolution. The recorded diffusion profiles reveal root-mean-square diffusion lengths for non-fluorescing and non-absorbing molecules, both small (glucose, 180 Da) and large (bovine serum albumin, 67 kDa).

SUBMITTER: Tolbol Sorensen K 

PROVIDER: S-EPMC6190349 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Label-Free Monitoring of Diffusion in Microfluidics.

Tølbøl Sørensen Kristian K   Kristensen Anders A  

Micromachines 20171109 11


Label-free, real-time detection of concentration gradients is demonstrated in a microfluidic H-filter, using an integrated photonic crystal slab sensor to monitor sample refractive index with spatial resolution. The recorded diffusion profiles reveal root-mean-square diffusion lengths for non-fluorescing and non-absorbing molecules, both small (glucose, 180 Da) and large (bovine serum albumin, 67 kDa). ...[more]

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