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A long noncoding RNA, lincRNA-Tnfaip3, acts as a coregulator of NF-?B to modulate inflammatory gene transcription in mouse macrophages.

ABSTRACT: Long intergenic noncoding RNAs (lincRNAs) are long noncoding transcripts (>200 nt) from the intergenic regions of annotated protein-coding genes. We report here that the lincRNA gene lincRNA-Tnfaip3, located at mouse chromosome 10 proximal to the tumor necrosis factor ?-induced protein 3 (Tnfaip3) gene, is an early-primary response gene controlled by nuclear factor-?B (NF-?B) signaling in murine macrophages. Functionally, lincRNA- Tnfaip3 appears to mediate both the activation and repression of distinct classes of inflammatory genes in macrophages. Specifically, induction of lincRNA-Tnfaip3 is required for the transactivation of NF-?B-regulated inflammatory genes in response to bacterial LPSs stimulation. LincRNA-Tnfaip3 physically interacts with the high-mobility group box 1 (Hmgb1), assembling a NF-?B/Hmgb1/lincRNA-Tnfaip3 complex in macrophages after LPS stimulation. This resultant NF-?B/Hmgb1/lincRNA-Tnfaip3 complex can modulate Hmgb1-associated histone modifications and, ultimately, transactivation of inflammatory genes in mouse macrophages in response to microbial challenge. Therefore, our data indicate a new regulatory role of NF-?B-induced lincRNA-Tnfaip3 to act as a coactivator of NF-?B for the transcription of inflammatory genes in innate immune cells through modulation of epigenetic chromatin remodeling.-Ma, S., Ming, Z., Gong, A.-Y., Wang, Y., Chen, X., Hu, G., Zhou, R., Shibata, A., Swanson, P. C., Chen, X.-M. A long noncoding RNA, LincRNA-Tnfaip3, acts as a coregulator of NF-?B to modulate inflammatory gene transcription in mouse macrophages.

SUBMITTER: Ma S

PROVIDER: S-EPMC6191062 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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A long noncoding RNA, lincRNA-Tnfaip3, acts as a coregulator of NF-κB to modulate inflammatory gene transcription in mouse macrophages.

Ma Shibin S Ming Zhenping Z Gong Ai-Yu AY Wang Yang Y Chen Xiqiang X Hu Guoku G Zhou Rui R Shibata Annemarie A Swanson Patrick C PC Chen Xian-Ming XM

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20161215 3

Long intergenic noncoding RNAs (lincRNAs) are long noncoding transcripts (>200 nt) from the intergenic regions of annotated protein-coding genes. We report here that the lincRNA gene <i>lincRNA-Tnfaip3</i>, located at mouse chromosome 10 proximal to the tumor necrosis factor α-induced protein 3 (<i>Tnfaip3</i>) gene, is an early-primary response gene controlled by nuclear factor-κB (NF-κB) signaling in murine macrophages. Functionally, lincRNA- Tnfaip3 appears to mediate both the activation and ...[more]

PMID: 27979905

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Dataset Information

A long noncoding RNA, lincRNA-Tnfaip3, acts as a coregulator of NF-?B to modulate inflammatory gene transcription in mouse macrophages.

Publications

A long noncoding RNA, lincRNA-Tnfaip3, acts as a coregulator of NF-κB to modulate inflammatory gene transcription in mouse macrophages.

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