M¹A within cytoplasmic mRNAs at single nucleotide resolution: a reconciled transcriptome-wide map.

ABSTRACT: Following synthesis, RNA can be modified with over 100 chemically distinct modifications. Recently, two studies-one by our group-developed conceptually similar approaches to globally map N1-methyladenosine (m¹A) at single nucleotide resolution. Surprisingly, the studies diverged quite substantially in their estimates of the abundance, whereabouts, and stoichiometry of m¹A within internal sites in cytosolic mRNAs: One study reported it to be a very rare modification, present at very low stoichiometries, and invariably catalyzed by TRMT6/61A. The other found it to be present at >470 sites, often at high levels, and suggested that the vast majority were highly unlikely to be TRMT6/61A substrates. Here we reanalyze the data from the latter study, and demonstrate that the

SUBMITTER: Schwartz S 

PROVIDER: S-EPMC6191711 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature