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A novel pH-dependent membrane peptide that binds to EphA2 and inhibits cell migration.

ABSTRACT: Misregulation of the signaling axis formed by the receptor tyrosine kinase (RTK) EphA2 and its ligand, ephrinA1, causes aberrant cell-cell contacts that contribute to metastasis. Solid tumors are characterized by an acidic extracellular medium. We intend to take advantage of this tumor feature to design new molecules that specifically target tumors. We created a novel pH-dependent transmembrane peptide, TYPE7, by altering the sequence of the transmembrane domain of EphA2. TYPE7 is highly soluble and interacts with the surface of lipid membranes at neutral pH, while acidity triggers transmembrane insertion. TYPE7 binds to endogenous EphA2 and reduces Akt phosphorylation and cell migration as effectively as ephrinA1. Interestingly, we found large differences in juxtamembrane tyrosine phosphorylation and the extent of EphA2 clustering when comparing TYPE7 with activation by ephrinA1. This work shows that it is possible to design new pH-triggered membrane peptides to activate RTK and gain insights on its activation mechanism.

SUBMITTER: Alves DS

PROVIDER: S-EPMC6192698 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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A novel pH-dependent membrane peptide that binds to EphA2 and inhibits cell migration.

Alves Daiane S DS Westerfield Justin M JM Shi Xiaojun X Nguyen Vanessa P VP Stefanski Katherine M KM Booth Kristen R KR Kim Soyeon S Morrell-Falvey Jennifer J Wang Bing-Cheng BC Abel Steven M SM Smith Adam W AW Barrera Francisco N FN

eLife 20180917

Misregulation of the signaling axis formed by the receptor tyrosine kinase (RTK) EphA2 and its ligand, ephrinA1, causes aberrant cell-cell contacts that contribute to metastasis. Solid tumors are characterized by an acidic extracellular medium. We intend to take advantage of this tumor feature to design new molecules that specifically target tumors. We created a novel pH-dependent transmembrane peptide, TYPE7, by altering the sequence of the transmembrane domain of EphA2. TYPE7 is highly soluble ...[more]

PMID: 30222105

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Dataset Information

A novel pH-dependent membrane peptide that binds to EphA2 and inhibits cell migration.

Publications

A novel pH-dependent membrane peptide that binds to EphA2 and inhibits cell migration.

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