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CRISPR-induced exon skipping is dependent on premature termination codon mutations.

ABSTRACT: In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered is controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of exon skipping at high frequency in premature termination codon-mutated rabbits but not in the rabbits with a premature termination codon mutation in exon 1 rabbits with non-frameshift or missense mutations. Our results suggest that CRISPR-mediated exon skipping depends on premature termination codon mutation-induced nonsense-associated altered splicing.

SUBMITTER: Sui T

PROVIDER: S-EPMC6193291 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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CRISPR-induced exon skipping is dependent on premature termination codon mutations.

Sui Tingting T Song Yuning Y Liu Zhiquan Z Chen Mao M Deng Jichao J Xu Yuanyuan Y Lai Liangxue L Li Zhanjun Z

Genome biology 20181017 1

In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered is controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of exon skipping at high frequency in premature termination codon-mutated rabbits but not in the rabbits with a premature termination codon mutation in exon 1 rabbits with non-frameshift or missense mutations. Our results suggest that CRISPR-mediated e ...[more]

PMID: 30333044

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