Ontology highlight
ABSTRACT:
SUBMITTER: Theint T
PROVIDER: S-EPMC6193843 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Theint Theint T Xia Yongjie Y Nadaud Philippe S PS Mukhopadhyay Dwaipayan D Schwieters Charles D CD Surewicz Krystyna K Surewicz Witold K WK Jaroniec Christopher P CP
Journal of the American Chemical Society 20181009 41
Application of paramagnetic solid-state NMR to amyloids is demonstrated, using Y145Stop human prion protein modified with nitroxide spin-label or EDTA-Cu<sup>2+</sup> tags as a model. By using sample preparation protocols based on seeding with preformed fibrils, we show that paramagnetic protein analogs can be induced into adopting the wild-type amyloid structure. Measurements of residue-specific intramolecular and intermolecular paramagnetic relaxation enhancements enable determination of prote ...[more]