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Statistical optimization of light intensity and CO₂ concentration for lipid production derived from attached cultivation of green microalga Ettlia sp.

ABSTRACT: Attached cultivation systems have been receiving extensive attention as a breakthrough in microalgae cultivation technology. However, there is a lack of studies that emphasize precise optimization of important parameters in attached cultivation of microalgae. In this study, the effects of two major environmental parameters in photoautotrophic cultivation, light intensity and CO₂ concentration, on the biomass and lipid surface productivity of Ettlia sp. YC001 were optimized by employing Response Surface Methodology (RSM) and validated experimentally. The optimum initial conditions for attached cultivation were use of seed from the late exponential phase (LE) and an inoculum surface density of 2.5 g/m². By optimization, maximum biomass surface productivity of 28.0 ± 1.5 g/m²/day was achieved at 730 μE/m²/s with 8% CO₂. The maximum lipid surface productivity was 4.2 ± 0.3 g/m²/day at 500 μE/m²/s with 7% CO₂. Change of the fatty acid composition with respect to changes in environment parameters led to improvement of biodiesel quality at higher light intensity and higher CO₂ concentration. Attached cultivation of Ettlia sp. YC001 has successfully produced biomass and lipids at a high production rate with relatively low light energy demand and high CO₂ utilization.

SUBMITTER: Kim S

PROVIDER: S-EPMC6193934 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BackgroundMarine microalgae are among the most promising lipid sources for biodiesel production because they can be grown on nonarable land without the use of potable water. Marine microalgae also harvest solar energy efficiently with a high growth rate, converting CO2 into lipids stored in the cells. Both light intensity and nitrogen availability strongly affect the growth, lipid accumulation, and fatty acid composition of oleaginous microalgae. However, very few studies have systematically examined how to optimize lipid productivity by adjusting irradiance intensity, and the metabolic dynamics that may lead to improved lipid accumulation in microalgae have not been elucidated. Little is known about the mechanism of lipid synthesis regulation in microalgae. Moreover, few studies have assessed the potential of using marine microalgae as oil producers.ResultsIn this work, a newly isolated marine microalga, Chlamydomonas sp. JSC4, was selected as a potential lipid producer, and the effect of photobioreactor operations on cell growth and lipid production was investigated. The combined effects of light intensity and nitrogen depletion stresses on growth and lipid accumulation were further explored in an effort to markedly improve lipid production and quality. The optimal lipid productivity and content attained were 312 mg L(-1) d(-1) and 43.1% per unit dry cell weight, respectively. This lipid productivity is the highest ever reported for marine microalgae. Metabolic intermediates were profiled over time to observe transient changes during lipid accumulation triggered by combined stresses. Finally, metabolite turnover was also assessed using an in vivo (13)C-labeling technique to directly measure the flow of carbon during lipid biosynthesis under stress associated with light intensity and nitrogen deficiency.ConclusionsThis work demonstrates the synergistic integration of cultivation and dynamic metabolic profiling technologies to develop a simple and effective strategy for enhancing oil production in a marine microalga. The knowledge obtained from this study could be useful in assessing the feasibility of marine microalgae biodiesel production and for understanding the links between dynamic metabolic profiles and lipid biosynthesis during the course of microalgal cultivation.

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Statistical optimization of light intensity and CO₂ concentration for lipid production derived from attached cultivation of green microalga Ettlia sp.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Statistical optimization of light intensity and CO2 concentration for lipid production derived from attached cultivation of green microalga Ettlia sp.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Statistical optimization of light intensity and CO₂ concentration for lipid production derived from attached cultivation of green microalga Ettlia sp.