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FtsZ filaments have the opposite kinetic polarity of microtubules.


ABSTRACT: FtsZ is the ancestral homolog of tubulin and assembles into the Z ring that organizes the division machinery to drive cell division in most bacteria. In contrast to tubulin that assembles into 13 stranded microtubules that undergo dynamic instability, FtsZ assembles into single-stranded filaments that treadmill to distribute the peptidoglycan synthetic machinery at the septum. Here, using longitudinal interface mutants of FtsZ, we demonstrate that the kinetic polarity of FtsZ filaments is opposite to that of microtubules. A conformational switch accompanying the assembly of FtsZ generates the kinetic polarity of FtsZ filaments, which explains the toxicity of interface mutants that function as a capper and reveals the mechanism of cooperative assembly. This approach can also be employed to determine the kinetic polarity of other filament-forming proteins.

SUBMITTER: Du S 

PROVIDER: S-EPMC6196554 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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FtsZ filaments have the opposite kinetic polarity of microtubules.

Du Shishen S   Pichoff Sebastien S   Kruse Karsten K   Lutkenhaus Joe J  

Proceedings of the National Academy of Sciences of the United States of America 20181001 42


FtsZ is the ancestral homolog of tubulin and assembles into the Z ring that organizes the division machinery to drive cell division in most bacteria. In contrast to tubulin that assembles into 13 stranded microtubules that undergo dynamic instability, FtsZ assembles into single-stranded filaments that treadmill to distribute the peptidoglycan synthetic machinery at the septum. Here, using longitudinal interface mutants of FtsZ, we demonstrate that the kinetic polarity of FtsZ filaments is opposi  ...[more]

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