Ontology highlight
ABSTRACT:
SUBMITTER: Peng X
PROVIDER: S-EPMC6197854 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Peng Xi X Emiliani Francesco F Smallwood Philip M PM Rattner Amir A Lei Hong H Sabbagh Mark F MF Nathans Jeremy J
eLife 20181022
Defining protein-protein interactions (PPIs) is central to the biological sciences. Here, we present a novel platform - Affinity Capture of Polyribosomes followed by RNA sequencing (ACAPseq) - for identifying PPIs. ACAPseq harnesses the power of massively parallel RNA sequencing (RNAseq) to quantify the enrichment of polyribosomes based on the affinity of their associated nascent polypeptides for an immobilized protein 'bait'. This method was developed and tested using neonatal mouse brain polyr ...[more]