Unknown

Dataset Information

0

Differential genome-wide profiling of alternative polyadenylation sites in nasopharyngeal carcinoma by high-throughput sequencing.


ABSTRACT: BACKGROUND:Alternative polyadenylation (APA) is a widespread phenomenon in the posttranscriptional regulation of gene expression that generates mRNAs with alternative 3'-untranslated regions (3'UTRs). APA contributes to the pathogenesis of various diseases, including cancer. However, the potential role of APA in the development of nasopharyngeal carcinoma (NPC) remains largely unknown. METHODS:A strategy of sequencing APA sites (SAPAS) based on second-generation sequencing technology was carried out to explore the global patterns of APA sites and identify genes with tandem 3'UTRs in samples from 6 NPC and 6 normal nasopharyngeal epithelial tissue (NNET). Sequencing results were then validated using quantitative RT-PCR in a larger cohort of 16 NPC and 16 NNET samples. RESULTS:The sequencing data showed that the use of tandem APA sites was prevalent in NPC, and numerous genes with APA-switching events were discovered. In total, we identified 195 genes with significant differences in the tandem 3'UTR length between NPC and NNET; including 119 genes switching to distal poly (A) sites and 76 genes switching to proximal poly (A) sites. Several gene ontology (GO) terms were enriched in the list of genes with switched APA sites, including regulation of cell migration, macromolecule catabolic process, protein catabolic process, proteolysis, small conjugating protein ligase activity, and ubiquitin-protein ligase activity. CONCLUSIONS:APA site-switching events are prevalent in NPC. APA-mediated regulation of gene expression may play an important role in the development of NPC, and more detailed studies targeting genes with APA-switching events may contribute to the development of novel future therapeutic strategies for NPC.

SUBMITTER: Xu YF 

PROVIDER: S-EPMC6198351 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Differential genome-wide profiling of alternative polyadenylation sites in nasopharyngeal carcinoma by high-throughput sequencing.

Xu Ya-Fei YF   Li Ying-Qing YQ   Liu Na N   He Qing-Mei QM   Tang Xin-Ran XR   Wen Xin X   Yang Xiao-Jing XJ   Sun Ying Y   Ma Jun J   Tang Ling-Long LL  

Journal of biomedical science 20181023 1


<h4>Background</h4>Alternative polyadenylation (APA) is a widespread phenomenon in the posttranscriptional regulation of gene expression that generates mRNAs with alternative 3'-untranslated regions (3'UTRs). APA contributes to the pathogenesis of various diseases, including cancer. However, the potential role of APA in the development of nasopharyngeal carcinoma (NPC) remains largely unknown.<h4>Methods</h4>A strategy of sequencing APA sites (SAPAS) based on second-generation sequencing technol  ...[more]

Similar Datasets

| S-EPMC6126484 | biostudies-literature
| S-EPMC3817161 | biostudies-literature
| S-EPMC4692544 | biostudies-literature
| S-EPMC4249744 | biostudies-literature
| S-EPMC10013925 | biostudies-literature
| S-EPMC6686930 | biostudies-literature
| S-EPMC6017670 | biostudies-literature
| S-EPMC7955126 | biostudies-literature
| S-EPMC6686416 | biostudies-literature
2020-02-01 | GSE110063 | GEO