Dataset Information

The Biogeographical Distribution of Soil Bacterial Communities in the Loess Plateau as Revealed by High-Throughput Sequencing.

ABSTRACT: The rigorous environmental stress of the severely eroded Loess Plateau may have promoted specific soil bacterial communities in comparison to other eco-environmental regions. In order to unmask the bacterial diversity and most influential environmental parameters, Illumina MiSeq high throughput sequencing of 16S rRNA from 24 representative soil samples collected across south-east to north-west transect of the Loess Plateau in northern Shaanxi, China was conducted. This high-throughput sequencing revealed a total of 1,411,001 high quality sequences that classified into 38 phyla, 127 classes, >240 orders, and over 650 genera, suggesting a high bacterial richness across the Loess Plateau soils. The seven dominant groups were: Proteobacteria, Actinobacteria, Acidobacteria, Planctomycetes, Gemmatimonadetes, Chloroflexi, and Verrucomicrobi (relative abundance of >5%). Increasing/decreasing soil pH and geographic longitudinal distance correlated significantly with increasing/decreasing bacterial richness and diversity indices. Pairwise correlation analysis showed higher bacterial diversity at longitudinal gradients across 107°39'-109°15' (south-east to north-west) in our studied Chinese loess zone. Variation partitioning analysis indicated significant influence of soil characteristics (~40.4%) than geographical distance (at a landscape scale of ~400 km) that was responsible for 13.6% of variation in bacterial community structure from these soils. Overall, contemporary soil characteristics structure the bacterial community in Loess Plateau soil to a greater extent than the spatial distances along the loess transect.

SUBMITTER: Liu D

PROVIDER: S-EPMC6200921 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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The Biogeographical Distribution of Soil Bacterial Communities in the Loess Plateau as Revealed by High-Throughput Sequencing.

Liu Dong D Yang Yang Y An Shaoshan S Wang Honglei H Wang Ying Y

Frontiers in microbiology 20181018

The rigorous environmental stress of the severely eroded Loess Plateau may have promoted specific soil bacterial communities in comparison to other eco-environmental regions. In order to unmask the bacterial diversity and most influential environmental parameters, Illumina MiSeq high throughput sequencing of 16S rRNA from 24 representative soil samples collected across south-east to north-west transect of the Loess Plateau in northern Shaanxi, China was conducted. This high-throughput sequencing ...[more]

PMID: 30405547

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Project description:Biochar application can enhance soil health and alter soil bacterial community structure. However, knowledge relating to biochar on soil nutrients of mountainous apple orchards and then assessing its effect on soil health, especially on soil microorganisms, is still scanty. Therefore, we evaluated the responses of six biochar treatments [Ck (0), T1 (2), T2 (4), T3 (6), T4 (8), and T5 (10) Mg hm-2] with a basal dose of chemical fertilizer on the soil nutrients under potted apple trees across 3, 6, 9, and 12 months, and then investigated the responses of the rhizobacterial communities. Experimental findings demonstrated that: (i) Across the months, the biochar-applied treatment (T5) compared to the control significantly enhanced soil nutrients, including soil pH (2.12 to 2.29%), soil organic matter (35 to 40%), total nitrogen (59 to 65%), ammonium nitrogen (25 to 33%), nitrate nitrogen (163 to 169%), and the activities of urease (76 to 81%), alkaline phosphatase (30 to 33%), catalase (8.89 to 11.70%), and sucrase (23 to 29%). (ii) Compared to the control, the biochar-applied treatment (T5) had a more desirable relative abundance of the bacterial phylum Proteobacteria (35.47%), followed by Actinobacteria (8.59%), Firmicutes (5.74%), and Bacteroidota (2.77%). Similarly, the relative abundance of the bacterial genera in the T5 was Sphingomonas (8.23%) followed by RB41 (3.81%), Ellin6055 (3.42%), Lachnospiracea (1.61%), Bacillus (1.43%), Kineosporia (1.37%), Massilia (0.84%), and Odoribacter (0.34%) than the control. (iii) Among the alpha diversity, the biochar-applied treatment (T5) revealed the highest Chao1 (20%) and ACE (19.23%) indexes, while Shannon (1.63%) and Simpson (1.02%) had relatively lower indexes than the control. Furthermore, positive correlations were found between the soil nutrients and some of the abundant bacterial phyla. Overall, the findings of this research demonstrated that biochar application at 10 Mg hm-2 (T5) along with the required chemical fertilizer is beneficial to improve soil health and pave the way for sustainable production in apple orchards of the northern loess plateau.

Dataset Information

The Biogeographical Distribution of Soil Bacterial Communities in the Loess Plateau as Revealed by High-Throughput Sequencing.

Publications

The Biogeographical Distribution of Soil Bacterial Communities in the Loess Plateau as Revealed by High-Throughput Sequencing.

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