Dataset Information

Impacts of Paraburkholderia phytofirmans Strain PsJN on Tomato (Lycopersicon esculentum L.) Under High Temperature.

ABSTRACT: Abnormal temperatures induce physiological and biochemical changes resulting in the loss of yield. The present study investigates the impact of the PsJN strain of Paraburkholderia phytofirmans on tomato (Lycopersicon esculentum Mill.) in response to heat stress (32°C). The results of this work showed that bacterial inoculation with P. phytofirmans strain PsJN increased tomato growth parameters such as chlorophyll content and gas exchange at both normal and high temperatures (25 and 32°C). At normal temperature (25°C), the rate of photosynthesis and the photosystem II activity increased with significant accumulations of sugars, total amino acids, proline, and malate in the bacterized tomato plants, demonstrating that the PsJN strain had a positive effect on plant growth. However, the amount of sucrose, total amino acids, proline, and malate were significantly affected in tomato leaves at 32°C compared to that at 25°C. Changes in photosynthesis and chlorophyll fluorescence showed that the bacterized tomato plants were well acclimated at 32°C. These results reinforce the current knowledge about the PsJN strain of P. phytofirmans and highlight in particular its ability to alleviate the harmful effects of high temperatures by stimulating the growth and tolerance of tomato plants.

SUBMITTER: Issa A

PROVIDER: S-EPMC6201190 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Impacts of Paraburkholderia phytofirmans Strain PsJN on Tomato (Lycopersicon esculentum L.) Under High Temperature.

Issa Alaa A Esmaeel Qassim Q Sanchez Lisa L Courteaux Barbara B Guise Jean-Francois JF Gibon Yves Y Ballias Patricia P Clément Christophe C Jacquard Cédric C Vaillant-Gaveau Nathalie N Aït Barka Essaïd E

Frontiers in plant science 20181018

Abnormal temperatures induce physiological and biochemical changes resulting in the loss of yield. The present study investigates the impact of the PsJN strain of Paraburkholderia phytofirmans on tomato (Lycopersicon esculentum Mill.) in response to heat stress (32°C). The results of this work showed that bacterial inoculation with P. phytofirmans strain PsJN increased tomato growth parameters such as chlorophyll content and gas exchange at both normal and high temperatures ...[more]

PMID: 30405648

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Project description:Plant defense responses to biotic stresses are complex biological processes, all governed by sophisticated molecular regulations. Induced systemic resistance (ISR) is one of these defense mechanisms where beneficial bacteria or fungi prime plants to resist pathogens or pest attacks. In ISR, the defense arsenal in plants remains dormant and it is only triggered by an infection, allowing a better allocation of plant resources. Our group recently described that the well-known beneficial bacterium Paraburkholderia phytofirmans PsJN is able to induce Arabidopsis thaliana resistance to Pseudomonas syringae pv. tomato (Pst) DC3000 through ISR, and that ethylene, jasmonate and salicylic acid are involved in this protection. Nevertheless, the molecular networks governing this beneficial interaction remain unknown. To tackle this issue, we analyzed the temporal changes in the transcriptome of PsJN-inoculated plants before and after being infected with Pst DC3000. These data were used to perform a gene network analysis to identify highly connected transcription factors. Before the pathogen challenge, the strain PsJN regulated 405 genes (corresponding to 1.8% of the analyzed genome). PsJN-inoculated plants presented a faster and stronger transcriptional response at 1-hour post infection (hpi) compared with the non-inoculated plants, which presented the highest transcriptional changes at 24 hpi. A principal component analysis showed that PsJN-induced plant responses to the pathogen could be differentiated from those induced by the pathogen itself. Forty-eight transcription factors were regulated by PsJN at 1 hpi, and a system biology analysis revealed a network with four clusters. Within these clusters LHY, WRKY28, MYB31 and RRTF1 are highly connected transcription factors, which could act as hub regulators in this interaction. Concordantly with our previous results, these clusters are related to jasmonate, ethylene, salicylic, acid and ROS pathways. These results indicate that a rapid and specific response of PsJN-inoculated plants to the virulent DC3000 strain could be the pivotal element in the protection mechanism.

Project description:Plant defense responses to biotic stresses are complex biological processes, all governed by sophisticated molecular regulations. Induced systemic resistance (ISR) is one of these defense mechanisms where beneficial bacteria or fungi prime plants to resist pathogen or pest attacks. In ISR the defense arsenal in plants remains dormant and it is only triggered by the infection, allowing a rapid response and a better allocation of plant resources. We recently described that the well-known beneficial bacterium Paraburkholderia phytofirmans PsJN is able to reduce Arabidopsis thaliana susceptibility to Pseudomonas syringae Pst DC3000 through ISR. Nevertheless, the molecular networks governing this beneficial interaction remain unknown. To tackle this issue we analyzed the temporal changes in the transcriptome of PsJN-inoculated plants before and after the infection with Pst DC3000 strain. These data were used to perform a gene network analysis to identify highly connected transcription factors that may be acting as hubs in controlling this ISR response. Before the pathogen challenge, strain PsJN regulated 405 genes (corresponding to 1.5% of the analyzed genome). PsJN-inoculated plants presented a faster and stronger transcriptional response 1-hour post infection (hpi) compared with the non-inoculated plants, which presented the highest transcriptional changes 24 hpi. A principal component analysis showed that PsJN-induced plant responses to the pathogen could be differentiated from those induced by the sole pathogen. Forty-eight transcription factors were regulated by PsJN 1 hpi, and a system biology analysis revealed a network with 4 modules where LHY, WRKY28, MYB31 and RRTF1 are highly connected transcription factors, that can be acting as hub regulators in this interaction. These modules are related to Jasmonate, Ethylene, Salicylic acid and ROS pathways. Additionally, the down- and up-regulation of ANAC32 and ORA59, respectively, support an important role of PDF1.2. These results indicate that a rapid and specific response of PsJN-inoculated plants to this virulent pathogen could be the pivotal element in the protection mechanism. A role for specific transcriptional regulators in the orchestration of this complex molecular response is also proposed

Dataset Information

Impacts of Paraburkholderia phytofirmans Strain PsJN on Tomato (Lycopersicon esculentum L.) Under High Temperature.

Publications

Impacts of <i>Paraburkholderia phytofirmans</i> Strain PsJN on Tomato (<i>Lycopersicon esculentum</i> L.) Under High Temperature.

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