Unknown

Dataset Information

0

The minor gentamicin complex component, X2, is a potent premature stop codon readthrough molecule with therapeutic potential.


ABSTRACT: Nonsense mutations, resulting in a premature stop codon in the open reading frame of mRNAs are responsible for thousands of inherited diseases. Readthrough of premature stop codons by small molecule drugs has emerged as a promising therapeutic approach to treat disorders resulting from premature termination of translation. The aminoglycoside antibiotics are a class of molecule known to promote readthrough at premature termination codons. Gentamicin consists of a mixture of major and minor aminoglycoside components. Here, we investigated the readthrough activities of the individual components and show that each of the four major gentamicin complex components representing 92-99% of the complex each had similar potency and activity to that of the complex itself. In contrast, a minor component (gentamicin X2) was found to be the most potent and active readthrough component in the gentamicin complex. The known oto- and nephrotoxicity associated with aminoglycosides preclude long-term use as readthrough agents. Thus, we evaluated the components of the gentamicin complex as well as the so-called "designer" aminoglycoside, NB124, for in vitro and in vivo safety. In cells, we observed that gentamicin X2 had a safety/readthrough ratio (cytotoxicity/readthrough potency) superior to that of gentamicin, G418 or NB124. In rodents, we observed that gentamicin X2 showed a safety profile that was superior to G418 overall including reduced nephrotoxicity. These results support further investigation of gentamicin X2 as a therapeutic readthrough agent.

SUBMITTER: Friesen WJ 

PROVIDER: S-EPMC6201930 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7237068 | biostudies-literature
| S-EPMC8053119 | biostudies-literature
| S-EPMC5304456 | biostudies-literature
| S-EPMC5100048 | biostudies-literature
| S-EPMC2982813 | biostudies-literature
2020-03-19 | PXD009401 | Pride
| S-EPMC9479597 | biostudies-literature
| S-EPMC5642019 | biostudies-literature
| S-EPMC10954755 | biostudies-literature
| S-EPMC154216 | biostudies-literature