Ontology highlight
ABSTRACT:
SUBMITTER: Tveriakhina L
PROVIDER: S-EPMC6202052 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Tveriakhina Lena L Schuster-Gossler Karin K Jarrett Sanchez M SM Andrawes Marie B MB Rohrbach Meike M Blacklow Stephen C SC Gossler Achim A
eLife 20181005
DLL1 and DLL4 are Notch ligands with high structural similarity but context-dependent functional differences. Here, we analyze their functional divergence using cellular co-culture assays, biochemical studies, and in vivo experiments. DLL1 and DLL4 activate NOTCH1 and NOTCH2 differently in cell-based assays and this discriminating potential lies in the region between the N-terminus and EGF repeat three. Mice expressing chimeric ligands indicate that the ectodomains dictate ligand function during ...[more]