Ontology highlight
ABSTRACT:
SUBMITTER: DiCara DM
PROVIDER: S-EPMC6204805 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
DiCara Danielle M DM Andersen Nisana N Chan Ruby R Ernst James A JA Ayalon Gai G Lazar Greg A GA Agard Nicholas J NJ Hilderbrand Amy A Hötzel Isidro I
mAbs 20180911 7
Developability assessment of therapeutic antibody candidates assists drug discovery by enabling early identification of undesirable instabilities. Rapid chemical stability screening of antibody variants can accelerate the identification of potential solutions. We describe here the development of a high-throughput assay to characterize asparagine deamidation. We applied the assay to identify a mutation that unexpectedly stabilizes a critical asparagine. Ninety antibody variants were incubated und ...[more]