Dataset Information

Cytotoxic and Bactericidal Effect of Silver Nanoparticles Obtained by Green Synthesis Method Using Annona muricata Aqueous Extract and Functionalized with 5-Fluorouracil.

ABSTRACT: Nanomaterials obtained by green synthesis technologies have been widely studied in recent years owing to constitute cost-effective and environmental-friendly methods. In addition, there are several works that report the simultaneous performance of the reducer agent as a functionalizing agent, modifying the properties of the nanomaterial. As a simple and economical synthesis methodology, this work presents a method to synthesize silver nanoparticles (AgNPs) using Annona muricata aqueous extract and functionalized with 5-fluorouracil (5-FU). The processes of reduction, nucleation, and functionalization of the nanoparticles were analyzed by UV-Vis absorption spectroscopy, and it was found that they are the function of the contact time of the metal ions with the extract. The structural characterization was carried out by transmission electron microscopy (TEM) and X-ray diffraction patterns (XRD). The antibacterial properties of the synthetized nanomaterials were tested using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Enterococcus faecalis, Staphylococcus aureus, and Escherichia coli growth.

SUBMITTER: Sanchez-Navarro MDC

PROVIDER: S-EPMC6205100 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Cytotoxic and Bactericidal Effect of Silver Nanoparticles Obtained by Green Synthesis Method Using <i>Annona muricata</i> Aqueous Extract and Functionalized with 5-Fluorouracil.

Sánchez-Navarro María Del Carmen MDC Ruiz-Torres Claudio Adrian CA Niño-Martínez Nereyda N Sánchez-Sánchez Roberto R Martínez-Castañón Gabriel Alejandro GA DeAlba-Montero I I Ruiz Facundo F

Bioinorganic chemistry and applications 20181015

Nanomaterials obtained by green synthesis technologies have been widely studied in recent years owing to constitute cost-effective and environmental-friendly methods. In addition, there are several works that report the simultaneous performance of the reducer agent as a functionalizing agent, modifying the properties of the nanomaterial. As a simple and economical synthesis methodology, this work presents a method to synthesize silver nanoparticles (AgNPs) using <i>Annona muricata</i> aqueous ex ...[more]

PMID: 30410535

Similar Datasets

Project description:This work reports the fast and effective bio-fabrication of novel platinum-palladium bimetallic nanoparticles (Pt-Pd BNPs) along with their counterparts Pt and Pd monometallic NPs (MNPs) through aqueous Annona muricata leaf extract. The bio-fabrication of the NPs was achieved within 2 h at 100 °C and pH 7 which was established by the occurrence of dark brown color for Pt MNPs and black color for Pd MNPs and Pt-Pd BNPs. NPs were evaluated for their catalytic activity in the reduction of methyl orange (MO), rhodamine-B (rh-B), and methylene blue (MB) textile dyes in presence of sodium borohydride as a reducing agent. Pt-Pd (1:3) BNPs showed higher MO dye degradation (96.84 ± 2.05% in 50 min) followed by Pd MNPs (97.07 ± 1.46% in 60 min), Pt-Pd (3:1) BNPs (97.34 ± 1.17% in 70 min) and Pt-Pd (1:1) BNPs (98.12 ± 1.04% in 80 min). Pd MNPs showed significant catalytic activity in the reduction of rh-B dye by 97.27 ± 1.14% in 12 min followed by Pt-Pd (3:1) BNPs (96.76 ± 2.17% in 18 min), Pt-Pd (1:3) BNPs (96.53 ± 1.97% in 33 min) and Pt-Pd (1:1) BNPs (97.11 ± 2.09% in 39 min). Pt-Pd (1:3) BNPs also showed higher MB dye degradation (96.95 ± 1.57% in 40 min) followed by Pd MNPs (96.22 ± 2.36% in 55 min), Pt-Pd (3:1) BNPs (97.29 ± 1.22% in 75 min) and Pt-Pd (1:1) BNPs (96.45 ± 2.19% in 105 min). However, Pt MNPs showed no catalytic activity. Standard disc diffusion method was used to evaluate the NPs toxicity towards Escherichia coli and Staphylococcus aureus, which showed no inhibitory zones. NPs showed less toxicity compared to potassium dichromate (control) against Artemia nauplii. Among the NPs studied, Pt-Pd (1:1) BNPs showed less toxicity with 100% mortality only at 100 µg/mL concentration followed by Pt MNPs (≥ 80 µg/mL), Pt-Pd (1:3) BNPs (≥ 60 µg/mL), Pt-Pd (3:1) BNPs (≥ 60 µg/mL) and Pd MNPs (≥ 40 µg/mL) after 72 h exposure. These evaluations support the application of bio-fabricated Pt-Pd BNPs as nano-catalysts in textile dyes degradation.Supplementary informationThe online version contains supplementary material available at 10.1007/s13205-021-02935-0.

Project description:BackgroundStaphylococcus aureus has prevailed against the majority of antibiotics currently in clinical use, making it a significant global public health problem. As a safer alternative, bioactive compounds have been explored. Annona muricata has been shown to possess antimicrobial activity. However, there are few reports on the molecular activity of A. muricata bioactive compounds against S. aureus. Thus, this study was aimed at evaluating the antimicrobial activity of its crude extract as well as investigating the potential of its bioactive compounds against the Cap5O capsular polysaccharides (CPS) of S. aureus via molecular docking.MethodsCollection of plant leaves, preparation of extracts, anti-nutrient analysis, phytochemical screening via crude method and gas chromatography-mass spectrophotometer (GC-MS), isolation and characterization of S. aureus and the antimicrobial activity test were all done using standard protocols. Molecular docking was done using the MCULE online tool with emphasis on docking scores, toxicity, and other properties.ResultsCrude screening of the extracts showed the presence of polyphenols, hydroxyanthraquinones, reducing compounds, flavonoids, saponins, glycosides, alkaloids, anthraquinones, phlobatannins and tannins in different concentrations. Anti-nutrient analysis showed the presence of allowable levels of evaluated anti-nutrients. GC-MS revealed a total of twenty-nine (29) bioactive compounds, out of which only 4 (13.80%) docked without toxicity and these were bicyclo[4.1.0]heptan-2-one 6-methyl, trichloromethane, carbonic acid 2-dimethylaminoethyl propyl ester, and 1-methyl-4-phenyl-5-thioxo-1,2,4-triazolidin-3-one on either the NAD-binding or C-terminal substrate binding domain of Cap5O.ConclusionResults obtained show that Cap5O could be a potential drug target for multi-drug resistant S. aureus, however, further studies aimed at evaluating these bioactive compounds individually and in combination are highly needed.

Dataset Information

Cytotoxic and Bactericidal Effect of Silver Nanoparticles Obtained by Green Synthesis Method Using Annona muricata Aqueous Extract and Functionalized with 5-Fluorouracil.

Publications

Cytotoxic and Bactericidal Effect of Silver Nanoparticles Obtained by Green Synthesis Method Using <i>Annona muricata</i> Aqueous Extract and Functionalized with 5-Fluorouracil.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets