Mechanically stimulated ATP release from murine bone cells is regulated by a balance of injury and repair.

ABSTRACT: Bone cells sense and actively adapt to physical perturbations to prevent critical damage. ATP release is among the earliest cellular responses to mechanical stimulation. Mechanical stimulation of a single murine osteoblast led to the release of 70 ± 24 amole ATP, which stimulated calcium responses in neighboring cells. Osteoblasts contained ATP-rich vesicles that were released upon mechanical stimulation. Surprisingly, interventions that promoted vesicular release reduced ATP release, while inhibitors of vesicular release potentiated ATP release. Searching for an alternative ATP release route, we found that mechanical stresses induced reversible cell membrane injury in vitro and in vivo. Ca²⁺/PLC/PKC-dependent vesicular exocytosis facilitated membrane repair, there

SUBMITTER: Mikolajewicz N 

PROVIDER: S-EPMC6205812 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature