Unknown

Dataset Information

0

Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis.


ABSTRACT: Non-coding RNAs regulate many biological processes including neurogenesis. The brain-enriched miR-124 has been assigned as a key player of neuronal differentiation via its complex but little understood regulation of thousands of annotated targets. To systematically chart its regulatory functions, we used CRISPR/Cas9 gene editing to disrupt all six miR-124 alleles in human induced pluripotent stem cells. Upon neuronal induction, miR-124-deleted cells underwent neurogenesis and became functional neurons, albeit with altered morphology and neurotransmitter specification. Using RNA-induced-silencing-complex precipitation, we identified 98 high-confidence miR-124 targets, of which some directly led to decreased viability. By performing advanced transcription-factor-network analysis, we identified indirect miR-124 effects on apoptosis, neuronal subtype differentiation, and the regulation of previously uncharacterized zinc finger transcription factors. Our data emphasize the need for combined experimental- and system-level analyses to comprehensively disentangle and reveal miRNA functions, including their involvement in the neurogenesis of diverse neuronal cell types found in the human brain.

SUBMITTER: Kutsche LK 

PROVIDER: S-EPMC6205824 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis.

Kutsche Lisa K LK   Gysi Deisy M DM   Fallmann Joerg J   Lenk Kerstin K   Petri Rebecca R   Swiersy Anka A   Klapper Simon D SD   Pircs Karolina K   Khattak Shahryar S   Stadler Peter F PF   Jakobsson Johan J   Nowick Katja K   Busskamp Volker V  

Cell systems 20181003 4


Non-coding RNAs regulate many biological processes including neurogenesis. The brain-enriched miR-124 has been assigned as a key player of neuronal differentiation via its complex but little understood regulation of thousands of annotated targets. To systematically chart its regulatory functions, we used CRISPR/Cas9 gene editing to disrupt all six miR-124 alleles in human induced pluripotent stem cells. Upon neuronal induction, miR-124-deleted cells underwent neurogenesis and became functional n  ...[more]

Similar Datasets

2018-10-08 | GSE118316 | GEO
2018-10-09 | GSE118315 | GEO
2018-10-08 | GSE118310 | GEO
2018-10-08 | GSE118307 | GEO
2018-10-08 | GSE118299 | GEO
2018-10-08 | GSE118302 | GEO
| PRJNA485153 | ENA
| PRJNA485155 | ENA
| PRJNA485148 | ENA
| PRJNA485136 | ENA