Advanced tube formation assay using human endothelial colony forming cells for in vitro evaluation of angiogenesis.

ABSTRACT: The tube formation assay is a widely used in vitro experiment model to evaluate angiogenic properties by measuring the formation of tubular structures from vascular endothelial cells (ECs). in vitro experimental results are crucial when considered the advisability of moving forward to in vivo studies. Thus, the additional attentions to the in vitro assay is necessary to improve the quality of the pre-clinical data, leading to better decision-making for successful drug discovery. In this study, we improved the tube formation assay system in three aspects. First, we used human endothelial colony forming cells (ECFCs), which are endothelial precursors that have a robust proliferative capacity and more defined angiogenic characteristics compared to mature ECs. Secon

SUBMITTER: Lee H 

PROVIDER: S-EPMC6205943 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature