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A Single-Cell Sequencing Guide for Immunologists.

ABSTRACT: In recent years there has been a rapid increase in the use of single-cell sequencing (scRNA-seq) approaches in the field of immunology. With the wide range of technologies available, it is becoming harder for users to select the best scRNA-seq protocol/platform to address their biological questions of interest. Here, we compared the advantages and limitations of four commonly used scRNA-seq platforms in order to clarify their suitability for different experimental applications. We also address how the datasets generated by different scRNA-seq platforms can be integrated, and how to identify unknown populations of single cells using unbiased bioinformatics methods.

SUBMITTER: See P

PROVIDER: S-EPMC6205970 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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A Single-Cell Sequencing Guide for Immunologists.

See Peter P Lum Josephine J Chen Jinmiao J Ginhoux Florent F

Frontiers in immunology 20181023

In recent years there has been a rapid increase in the use of single-cell sequencing (scRNA-seq) approaches in the field of immunology. With the wide range of technologies available, it is becoming harder for users to select the best scRNA-seq protocol/platform to address their biological questions of interest. Here, we compared the advantages and limitations of four commonly used scRNA-seq platforms in order to clarify their suitability for different experimental applications. We also address h ...[more]

PMID: 30405621

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