Dataset Information

Comparison of CAT-21A/T Gene Polymorphism in Women with Preeclampsia and Control Group.

ABSTRACT:

Background

Preeclampsia (PE) is one of the main causes of maternal and perinatal mortality and morbidity. Evidence shows that oxidative stress plays an important role in the pathophysiology of the PE. As catalase is a key enzyme in antioxidant enzymatic defense which protects cell from oxidative damage, in this study, we aimed to investigate the relationship between CAT-21A/T (rs7943316) polymorphism and PE susceptibility.

Materials and methods

This case-control study was conducted on 155 PE women and 159 normotensive pregnant women. Polymerase chain reaction-restriction fragment length polymorphism method was used for genotyping.

Results

There was no association between CAT-21AT and TT genotypes and PE susceptibility. However, the CAT-21A/T polymorphism was associated with 1.6-fold higher risk of PE in dominant model (AA vs. AT + TT) (odds ratio [OR] 1.6 [95% confidence interval [CI]: 0.9-2.9]; P = 0.04). However, the CAT-21A/T polymorphism was not associated with PE in recessive model (TT vs. AA + AT) (OR 1.3 [95% CI: 0.8-2.1]; P = 0.4).

Conclusions

The CAT-21A/T polymorphism could be a risk factor for PE susceptibility in dominant model.

SUBMITTER: Yassaee F

PROVIDER: S-EPMC6206746 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

Comparison of CAT-21A/T Gene Polymorphism in Women with Preeclampsia and Control Group.

Yassaee Fakhrolmolouk F Salimi Saeedeh S Etemadi Samaneh S Yaghmaei Minoo M

Advanced biomedical research 20181024

<h4>Background</h4>Preeclampsia (PE) is one of the main causes of maternal and perinatal mortality and morbidity. Evidence shows that oxidative stress plays an important role in the pathophysiology of the PE. As catalase is a key enzyme in antioxidant enzymatic defense which protects cell from oxidative damage, in this study, we aimed to investigate the relationship between CAT-21A/T (rs7943316) polymorphism and PE susceptibility.<h4>Materials and methods</h4>This case-control study was conducte ...[more]

PMID: 30464933

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Project description:Background Preeclampsia is a pregnancy-specific syndrome that may be dangerous especially to the fetus. Different cytokines have been found to be elevated in women with preeclampsia and may have possible roles in the development of this disorder, so the study was to designed to investigate the genetic polymorphism of IFN-γ (+874 A/T; rs2430561), TNF-α (−308 G/A; rs1800629), TNF-α (−238 rs361525), IL-4 (rs2243250), IL-6 rs1800795, IL-10 (−819) rs1800871, IL-10 (−1082) rs1800896, IL-10 (−592) rs1800872 with preeclampsia and healthy controls in North Coastal Andhra Pradesh of India. Methods A total of 200 samples (100 preeclamptic women and 100 normal pregnant women as control group) using allele-specific oligonucleotides-polymerase chain reaction and PCR RFLP method were genotyped. Data was analyzed using chi-square and Fisher’s exact tests. Results Three SNPs (TNF-α, IL-4 and IL-6) showed association with the genotype and allele frequencies between the study groups. TNF-α (G-308A) G/G genotype showed a significantly higher frequency among the preeclamptic group than the control group [odds ratio (OR): 0.4603, 95% confidence interval (CI): 0.2521–0.8405; P=0.005]. G/A genotype also showed higher frequency in both the study groups (OR: 2.508, 95% CI: 1.341–4.689; P=0.001). IL-4 (C590T) C/T genotype showed significantly higher frequency among the preeclamptic group compared to controls (OR: 2.452, 95% CI: 1.299–4.626; P=0.002), IL-6 (G174C) genotype significantly higher frequency among the preeclamptic group than the control group (OR: 0.4603, 95%: 0.2521–0.8405; P=0.005). There was no significant difference in genotype, allele frequencies and no independent association among the other three SNPs between the groups. Conclusions The present study might suggest a role for TNF-α (G-308A), IL-4 (C590T), −174 GC of IL-6 genotype in the development of preeclampsia; suggesting that they are of differing genetic predisposition/pathophysiology.

Project description:BACKGROUND:Leptin receptor gene (LEPR) variants may affect the leptin levels and act as a risk factor for preeclampsia. Two LEPR gene missense variants rs1137101 (c.668A>G) and rs1805094 (c.1968G>C) were investigated in Sudanese women with preeclampsia. METHODS:A matched case-control study (122 women in each arm) was conducted in Saad Abualila Maternity Hospital in Khartoum, Sudan from May to December 2018. The cases were women with preeclampsia and the controls were healthy pregnant women. Genotyping for LEPR gene variants c.668A>G and c.1968G>C was performed using polymerase chain reaction-restriction fragment length polymorphism. Logistic regression models (adjusted for age, parity, body mass index and hemoglobin level) were conducted. RESULTS:Genotype frequency of LEPR gene variants c.668A>G and c.1968G>C was in accordance with Hardy-Weinberg equilibrium (P > 0.05) in the controls. Allele G in LEPRc.668A>G variant was significantly more frequent in the cases compared with the controls [43.4% vs. 10.2%; OR = 6.44; 95%CI (3.98-10.40); P < 0.001]. In variant LEPRc.668A>G, genotype AG was the prevalent genotype in the cases compared with the controls, and it was significantly associated with preeclampsia risk [37.7% vs. 15.5%; AOR = 3.48; 95%CI (1.15-10.54); P = 0.027]. Likewise, the GG genotype was the second most common genotype in the cases compared with the controls, and was associated with preeclampsia risk [24.6% vs. 2.5%; AOR = 14.19; 95%CI (1.77-113.76); P = 0.012]. None of the LEPRc.1968G>C variant genotypes were associated with preeclampsia. The CC genotype was not detected in neither the cases nor the controls. The haplotype A-G 70.1% was the prevalent haplotype in this population, and it significantly protected against preeclampsia [OR = 0.14; 95%CI (0.09-0.23); P < 0.001]. However, the haplotype G-G 26.8% was significantly associated with preeclampsia risk [OR = 6.70; 95%CI (4.16-11.05); P < 0.001]. Both variants c.668A>G and c.1968G>C were in strong linkage disequilibrium (D' = 1, r2 = 0.012). CONCLUSIONS:Our data indicate that the rs1137101 (c.668A>G) variant and G-G haplotype may independently associate with the development of preeclampsia.

Dataset Information

Comparison of CAT-21A/T Gene Polymorphism in Women with Preeclampsia and Control Group.

Background

Materials and methods

Results

Conclusions

Publications

Comparison of CAT-21A/T Gene Polymorphism in Women with Preeclampsia and Control Group.

Similar Datasets

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