Paraneoplastic neuronal intermediate filament autoimmunity.

ABSTRACT:

Objective

To describe paraneoplastic neuronal intermediate filament (NIF) autoimmunity.

Methods

Archived patient and control serum and CSF specimens were evaluated by tissue-based indirect immunofluorescence assay (IFA). Autoantigens were identified by Western blot and mass spectrometry. NIF specificity was confirmed by dual tissue section staining and 5 recombinant NIF-specific HEK293 cell-based assays (CBAs, for α-internexin, neurofilament light [NfL], neurofilament medium, or neurofilament heavy chain, and peripherin). NIF-immunoglobulin Gs (IgGs) were correlated with neurologic syndromes and cancers.

Results

Among 65 patients, NIF-IgG-positive by IFA and CBAs, 33 were female (51%). Median symptom onset age was 62 years (range 18-88). Patients fell into 2 groups, d

SUBMITTER: Basal E 

PROVIDER: S-EPMC6207411 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature