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The structure of a ?2-microglobulin fibril suggests a molecular basis for its amyloid polymorphism.


ABSTRACT: All amyloid fibrils contain a cross-? fold. How this structure differs in fibrils formed from proteins associated with different diseases remains unclear. Here, we combine cryo-EM and MAS-NMR to determine the structure of an amyloid fibril formed in vitro from ?2-microglobulin (?2m), the culprit protein of dialysis-related amyloidosis. The fibril is composed of two identical protofilaments assembled from subunits that do not share ?2m's native tertiary fold, but are formed from similar ?-strands. The fibrils share motifs with other amyloid fibrils, but also contain unique features including ?-stacking interactions perpendicular to the fibril axis and an intramolecular disulfide that stabilises the subunit fold. We also describe a structural model for a second fibril morphology and show that it is built from the same subunit fold. The results provide insights into the mechanisms of fibril formation and the commonalities and differences within the amyloid fold in different protein sequences.

SUBMITTER: Iadanza MG 

PROVIDER: S-EPMC6207761 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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The structure of a β<sub>2</sub>-microglobulin fibril suggests a molecular basis for its amyloid polymorphism.

Iadanza Matthew G MG   Silvers Robert R   Boardman Joshua J   Smith Hugh I HI   Karamanos Theodoros K TK   Debelouchina Galia T GT   Su Yongchao Y   Griffin Robert G RG   Ranson Neil A NA   Radford Sheena E SE  

Nature communications 20181030 1


All amyloid fibrils contain a cross-β fold. How this structure differs in fibrils formed from proteins associated with different diseases remains unclear. Here, we combine cryo-EM and MAS-NMR to determine the structure of an amyloid fibril formed in vitro from β<sub>2</sub>-microglobulin (β<sub>2</sub>m), the culprit protein of dialysis-related amyloidosis. The fibril is composed of two identical protofilaments assembled from subunits that do not share β<sub>2</sub>m's native tertiary fold, but  ...[more]

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