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Disease-relevant transcriptional signatures identified in individual smooth muscle cells from healthy mouse vessels.

ABSTRACT: Vascular smooth muscle cells (VSMCs) show pronounced heterogeneity across and within vascular beds, with direct implications for their function in injury response and atherosclerosis. Here we combine single-cell transcriptomics with lineage tracing to examine VSMC heterogeneity in healthy mouse vessels. The transcriptional profiles of single VSMCs consistently reflect their region-specific developmental history and show heterogeneous expression of vascular disease-associated genes involved in inflammation, adhesion and migration. We detect a rare population of VSMC-lineage cells that express the multipotent progenitor marker Sca1, progressively downregulate contractile VSMC genes and upregulate genes associated with VSMC response to inflammation and growth factors. We find that Sca1 upregulation is a hallmark of VSMCs undergoing phenotypic switching in vitro and in vivo, and reveal an equivalent population of Sca1-positive VSMC-lineage cells in atherosclerotic plaques. Together, our analyses identify disease-relevant transcriptional signatures in VSMC-lineage cells in healthy blood vessels, with implications for disease susceptibility, diagnosis and prevention.

SUBMITTER: Dobnikar L

PROVIDER: S-EPMC6212435 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Disease-relevant transcriptional signatures identified in individual smooth muscle cells from healthy mouse vessels.

Dobnikar Lina L Taylor Annabel L AL Chappell Joel J Oldach Phoebe P Harman Jennifer L JL Oerton Erin E Dzierzak Elaine E Bennett Martin R MR Spivakov Mikhail M Jørgensen Helle F HF

Nature communications 20181101 1

Vascular smooth muscle cells (VSMCs) show pronounced heterogeneity across and within vascular beds, with direct implications for their function in injury response and atherosclerosis. Here we combine single-cell transcriptomics with lineage tracing to examine VSMC heterogeneity in healthy mouse vessels. The transcriptional profiles of single VSMCs consistently reflect their region-specific developmental history and show heterogeneous expression of vascular disease-associated genes involved in in ...[more]

PMID: 30385745

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Disease-relevant transcriptional signatures identified in individual smooth muscle cells from healthy mouse vessels.

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Disease-relevant transcriptional signatures identified in individual smooth muscle cells from healthy mouse vessels.

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