Dataset Information

Characterization of the Microbiome at the World's Largest Potable Water Reuse Facility.

ABSTRACT: Conventional water resources are not sufficient in many regions to meet the needs of growing populations. Due to cyclical weather cycles, drought, and climate change, water stress has increased worldwide including in Southern California, which serves as a model for regions that integrate reuse of wastewater for both potable and non-potable use. The Orange County Water District (OCWD) Advanced Water Purification Facility (AWPF) is a highly engineered system designed to treat and produce up to 100 million gallons per day (MGD) of purified water from a municipal wastewater source for potable reuse. Routine facility microbial water quality analysis is limited to standard indicators at this and similar facilities. Given recent advances in high throughput DNA sequencing techniques, complete microbial profiling of communities in water samples is now possible. By using 16S/18S rRNA gene sequencing, metagenomic and metatranscriptomic sequencing coupled to a highly accurate identification method along with 16S rRNA gene qPCR, we describe a detailed view of the total microbial community throughout the facility. The total bacterial load of the water at stages of the treatment train ranged from 3.02 × 106 copies in source, unchlorinated wastewater feed to 5.49 × 101 copies of 16S rRNA gene/mL after treatment (consisting of microfiltration, reverse osmosis, and ultraviolet/advanced oxidation). Microbial diversity and load decreased by several orders of magnitude after microfiltration and reverse osmosis treatment, falling to almost non-detectable levels that more closely resembled controls of molecular grade laboratory water than the biomass detected in the source water. The presence of antibiotic resistance genes and viruses was also greatly reduced. Overall, system design performance was achieved, and comprehensive microbial community analysis was found to enable a more complete characterization of the water/wastewater microbial signature.

SUBMITTER: Stamps BW

PROVIDER: S-EPMC6212505 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Characterization of the Microbiome at the World's Largest Potable Water Reuse Facility.

Stamps Blake W BW Leddy Menu B MB Plumlee Megan H MH Hasan Nur A NA Colwell Rita R RR Spear John R JR

Frontiers in microbiology 20181026

Conventional water resources are not sufficient in many regions to meet the needs of growing populations. Due to cyclical weather cycles, drought, and climate change, water stress has increased worldwide including in Southern California, which serves as a model for regions that integrate reuse of wastewater for both potable and non-potable use. The Orange County Water District (OCWD) Advanced Water Purification Facility (AWPF) is a highly engineered system designed to treat and produce up to 100 ...[more]

PMID: 30416489

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Project description:In the search for sustainable drinking water, many countries are weighing up the benefits of advanced treatment technologies as a proactive measure to assist with the transformation of treated wastewater into a source of water used for the production of potable water. We investigated the biological effects along a pilot plant with an advanced water treatment process, using zebrafish embryos at different stages of development. The study took an innovative approach, comparing phenotypic observations with whole genome responses. This enabled us to keep an open mind about which chemicals might be influencing the biological activity. There was no evidence of acute toxicity at any stage of treatment, but distinctive abnormalities – skeletal, cardiovascular and pigmentation – occurred in a small proportion of embryos along the treatment process, and in a tap water, that were not detected in the aquarium water control. Reverse osmosis (RO) reduced the concentration of measured chemical contaminants in the water the most, whilst eliminating the occurrence of abnormalities detected in the fish. In contrast, advanced oxidation appeared to reverse the benefits of RO treatment by increasing the frequency of teratogenic and sub-lethal abnormalities seen in embryos. Genomic analysis found alterations to the retinoid system, which was consistent with the teratogenic abnormalities observed. In addition, we found evidence of changes to metabolic pathways, including tryptophan metabolism associated with the production of melatonin required for the control of normal circadian rhythms. Although we cannot extrapolate these preliminary findings in zebrafish embryos to human or environmental health, we show that underexplored forms of biological activity (that existing Test Guidelines are not designed to capture) occur in treated wastewater effluent, and/or may be created depending on the type of advanced treatment process used. Although the identity of the culprit chemicals are unknown at this time, our innovative approach highlights the need for more research into the effects of chemicals on the retinoid system (and metabolism).

Project description:The data presented in this article were collected using a large-scale public survey distributed through the mail to a random sample of 4000 water utility residential account holders in Albuquerque, New Mexico, USA. The survey collected data on a variety of water-related topics, including water scarcity, climate change, water use at home, perceptions of water sources and water quality, conservation habits, level of acceptance of two potable water reuse scenarios, and level of trust in a variety of entities. The survey also collected demographic data from respondents. Account holders received one of four survey versions, three of which provided different sets of educational material to test different motivations for accepting potable water reuse, and one provided no educational material. The survey was designed and administered using the Tailored Design Method, which involved focus groups, individual debriefing sessions, and a pre-test with members of the sample population to refine the survey instrument, and included a system of five contacts mailed out over a period of several months to maximize response rate. Mail-in and electronic response options were available, and the response rate was 46% (n = 1831). The data were compiled using Survey Monkey and organized using Microsoft Excel and RStudio. The data set featured in this article provides raw survey data plus additional variables created by grouping and consolidating answer options in the raw data. This is the first and most comprehensive set of data known to the authors on public perceptions of water resources and reuse in an arid inland community, and the authors have published open access papers based on this data set, which are linked to this paper. Water managers, planners, engineers, and utilities may be interested in using the data as a point of comparison for their own study on community knowledge of water resources and acceptance of water reuse or in examining the data for relationships not yet explored in the literature.

Dataset Information

Characterization of the Microbiome at the World's Largest Potable Water Reuse Facility.

Publications

Characterization of the Microbiome at the World's Largest Potable Water Reuse Facility.

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