Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains.

ABSTRACT: An automated dual-resolution serial optical coherence tomography (2R-SOCT) scanner is developed. The serial histology system combines a low-resolution ( 25    μ m / voxel ) 3 × OCT with a high-resolution ( 1.5    μ m / voxel ) 40 × OCT to acquire whole mouse brains at low resolution and to target specific regions of interest (ROIs) at high resolution. The 40 × ROIs positions are selected either manually by the microscope operator or using an automated ROI positioning selection algorithm. Additionally, a multimodal and multiresolution registration pipeline is developed in order to align the 2R-SOCT data onto diffusion MRI (dMRI) data acquired in the same ex vivo mouse brains prior to automated histology. Using this imaging system, 3 whole mouse brains are imaged, and 250 high-re

SUBMITTER: Lefebvre J 

PROVIDER: S-EPMC6215086 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature