Dataset Information

Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains.

ABSTRACT: An automated dual-resolution serial optical coherence tomography (2R-SOCT) scanner is developed. The serial histology system combines a low-resolution ( 25???m/voxel ) 3× OCT with a high-resolution ( 1.5???m/voxel ) 40× OCT to acquire whole mouse brains at low resolution and to target specific regions of interest (ROIs) at high resolution. The 40× ROIs positions are selected either manually by the microscope operator or using an automated ROI positioning selection algorithm. Additionally, a multimodal and multiresolution registration pipeline is developed in order to align the 2R-SOCT data onto diffusion MRI (dMRI) data acquired in the same ex vivo mouse brains prior to automated histology. Using this imaging system, 3 whole mouse brains are imaged, and 250 high-resolution 40× three-dimensional ROIs are acquired. The capability of this system to perform multimodal imaging studies is demonstrated by labeling the ROIs using a mouse brain atlas and by categorizing the ROIs based on their associated dMRI measures. This reveals a good correspondence of the tissue microstructure imaged by the high-resolution OCT with various dMRI measures such as fractional anisotropy, number of fiber orientations, apparent fiber density, orientation dispersion, and intracellular volume fraction.

SUBMITTER: Lefebvre J

PROVIDER: S-EPMC6215086 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains.

Lefebvre Joël J Delafontaine-Martel Patrick P Pouliot Philippe P Girouard Hélène H Descoteaux Maxime M Lesage Frédéric F

Neurophotonics 20181001 4

An automated dual-resolution serial optical coherence tomography (2R-SOCT) scanner is developed. The serial histology system combines a low-resolution ( 25 μ m / voxel ) 3 × OCT with a high-resolution ( 1.5 μ m / voxel ) 40 × OCT to acquire whole mouse brains at low resolution and to target specific regions of interest (ROIs) at high resolution. The 40 × ROIs positions are selected either manually by the microscope operator or using an automated ROI positioning selection algorithm. Ad ...[more]

PMID: 30681668

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Dataset Information

Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains.

Publications

Fully automated dual-resolution serial optical coherence tomography aimed at diffusion MRI validation in whole mouse brains.

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