Dataset Information

MicroRNA profiling of diabetic atherosclerosis in a rat model.

ABSTRACT:

Objective

The incidence of diabetic atherosclerosis (DA) is increasing worldwide. The study aim was to identify differentially expressed microRNAs (DE-miRs) potentially associated with the initiation and/or progression of DA, thereby yielding new insights into this disease.

Methods

Matched iliac artery tissue samples were isolated from 6 male rats with or without DA. The Affymetrix GeneChip microRNA 4.0 Array was used to detect miRs. Differential expression between atherosclerotic group and non-atherosclerotic group samples was analyzed using the Gene-Cloud of Biotechnology Information platform. Targetscan and miRanda were then used to predict targets of DE-miRs. Functions and pathways were identified for significantly enriched candidate target genes and a DE-miR functional regulatory network was assembled to identify DA-associated core target genes.

Results

A total of nine DE-miRs (rno-miR-206-3p, rno-miR-133a-5p, rno-miR-133b-3p, rno-miR-133a-3p, rno-miR-325-5p, rno-miR-675-3p, rno-miR-411-5p, rno-miR-329-3p, and rno-miR-126a-3p) were identified, all of which were up-regulated and together predicted to target 3349 genes. The target genes were enriched in known functions and pathways related to lipid and glucose metabolism. The functional regulatory network indicated a modulatory pattern of these metabolic functions with DE-miRs. The miR-gene network suggested arpp19 and MDM4 as possible DA-related core target genes.

Conclusion

The present study identified DE-miRs and miRNA-gene networks enriched for lipid and glucose metabolic functions and pathways, and arpp19 and MDM4 as potential DA-related core target genes, suggesting DE-miRs and/or arpp19 and MDM4 could act as potential diagnostic markers or therapeutic targets for DA.

SUBMITTER: Li Y

PROVIDER: S-EPMC6215356 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Publications

MicroRNA profiling of diabetic atherosclerosis in a rat model.

Li Yuejin Y Xiao Le L Li Jinyuan J Sun Ping P Shang Lei L Zhang Jian J Zhao Quan Q Ouyang Yiming Y Li Linhai L Gong Kunmei K

European journal of medical research 20181103 1

<h4>Objective</h4>The incidence of diabetic atherosclerosis (DA) is increasing worldwide. The study aim was to identify differentially expressed microRNAs (DE-miRs) potentially associated with the initiation and/or progression of DA, thereby yielding new insights into this disease.<h4>Methods</h4>Matched iliac artery tissue samples were isolated from 6 male rats with or without DA. The Affymetrix GeneChip microRNA 4.0 Array was used to detect miRs. Differential expression between atherosclerotic ...[more]

PMID: 30390707

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Dataset Information

MicroRNA profiling of diabetic atherosclerosis in a rat model.

Objective

Methods

Results

Conclusion

Publications

MicroRNA profiling of diabetic atherosclerosis in a rat model.

Similar Datasets

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