Dataset Information

Norepinephrine triggers an immediate-early regulatory network response in primary human white adipocytes.

ABSTRACT:

Background

Norepinephrine (NE) signaling has a key role in white adipose tissue (WAT) functions, including lipolysis, free fatty acid liberation and, under certain conditions, conversion of white into brite (brown-in-white) adipocytes. However, acute effects of NE stimulation have not been described at the transcriptional network level.

Results

We used RNA-seq to uncover a broad transcriptional response. The inference of protein-protein and protein-DNA interaction networks allowed us to identify a set of immediate-early genes (IEGs) with high betweenness, validating our approach and suggesting a hierarchical control of transcriptional regulation. In addition, we identified a transcriptional regulatory network with IEGs as master regulators, including HSF1 and NFIL3 as novel NE-induced IEG candidates. Moreover, a functional enrichment analysis and gene clustering into functional modules suggest a crosstalk between metabolic, signaling, and immune responses.

Conclusions

Altogether, our network biology approach explores for the first time the immediate-early systems level response of human adipocytes to acute sympathetic activation, thereby providing a first network basis of early cell fate programs and crosstalks between metabolic and transcriptional networks required for proper WAT function.

SUBMITTER: Higareda-Almaraz JC

PROVIDER: S-EPMC6215669 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Publications

Norepinephrine triggers an immediate-early regulatory network response in primary human white adipocytes.

Higareda-Almaraz Juan Carlos JC Karbiener Michael M Giroud Maude M Pauler Florian M FM Gerhalter Teresa T Herzig Stephan S Scheideler Marcel M

BMC genomics 20181103 1

<h4>Background</h4>Norepinephrine (NE) signaling has a key role in white adipose tissue (WAT) functions, including lipolysis, free fatty acid liberation and, under certain conditions, conversion of white into brite (brown-in-white) adipocytes. However, acute effects of NE stimulation have not been described at the transcriptional network level.<h4>Results</h4>We used RNA-seq to uncover a broad transcriptional response. The inference of protein-protein and protein-DNA interaction networks allowed ...[more]

PMID: 30390616

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Dataset Information

Norepinephrine triggers an immediate-early regulatory network response in primary human white adipocytes.

Background

Results

Conclusions

Publications

Norepinephrine triggers an immediate-early regulatory network response in primary human white adipocytes.

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