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Dysregulated gene expression predicts tumor aggressiveness in African-American prostate cancer patients.

ABSTRACT: Molecular mechanisms underlying the health disparity of prostate cancer (PCa) have not been fully determined. In this study, we applied bioinformatic approach to identify and validate dysregulated genes associated with tumor aggressiveness in African American (AA) compared to Caucasian American (CA) men with PCa. We retrieved and analyzed microarray data from 619 PCa patients, 412 AA and 207 CA, and we validated these genes in tumor tissues and cell lines by Real-Time PCR, Western blot, immunocytochemistry (ICC) and immunohistochemistry (IHC) analyses. We identified 362 differentially expressed genes in AA men and involved in regulating signaling pathways associated with tumor aggressiveness. In PCa tissues and cells, NKX3.1, APPL2, TPD52, LTC4S, ALDH1A3 and AMD1 transcripts were significantly upregulated (p?

SUBMITTER: Ali HEA

PROVIDER: S-EPMC6218553 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Dysregulated gene expression predicts tumor aggressiveness in African-American prostate cancer patients.

Ali Hamdy E A HEA Lung Pei-Yau PY Sholl Andrew B AB Gad Shaimaa A SA Bustamante Juan J JJ Ali Hamed I HI Rhim Johng S JS Deep Gagan G Zhang Jinfeng J Abd Elmageed Zakaria Y ZY

Scientific reports 20181105 1

Molecular mechanisms underlying the health disparity of prostate cancer (PCa) have not been fully determined. In this study, we applied bioinformatic approach to identify and validate dysregulated genes associated with tumor aggressiveness in African American (AA) compared to Caucasian American (CA) men with PCa. We retrieved and analyzed microarray data from 619 PCa patients, 412 AA and 207 CA, and we validated these genes in tumor tissues and cell lines by Real-Time PCR, Western blot, immunocy ...[more]

PMID: 30397274

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