Ontology highlight
ABSTRACT:
SUBMITTER: Warris S
PROVIDER: S-EPMC6218980 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
BMC genomics 20181106 1
<h4>Background</h4>Next-generation sequencing requires sufficient DNA to be available. If limited, whole-genome amplification is applied to generate additional amounts of DNA. Such amplification often results in many chimeric DNA fragments, in particular artificial palindromic sequences, which limit the usefulness of long sequencing reads.<h4>Results</h4>Here, we present Pacasus, a tool for correcting such errors. Two datasets show that it markedly improves read mapping and de novo assembly, yie ...[more]