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Positive effect of ROR?t on the prognosis of thyroid papillary carcinoma patients combined with Hashimoto's thyroiditis.

ABSTRACT: OBJECTIVES:To investigate the positive effect of Th17 cells on the prognosis of patients with PTC and HT. METHODS:The expression of nuclear specific marker ROR?t of Th17 cells in fresh and paraffin thyroid tissues and serum specimens were analyzed. Flow cytometry was used to detect the formation rates of Th17 cells (CD3+CD8-IL-17A+/CD3+CD8-%) at different time points after co-culture of thyroid papillary carcinoma cell line (TPC-1 and K1) and umbilical cord blood initial T lymphocytes. The protein expression of ROR?t in T lymphocytes after co-culture was detected. Preoperative serum levels of Th17 (IL-17) cytokines were measured. RESULTS:The positive expression of ROR?t in the tumor microenvironment of PTC patients with or without HT could inhibit the lymph node metastasis of the tumor. PTC cancer cells could induce initial T lymphocyte to differentiate into Th17 cells, and the K1 cell line with lymph node metastasis induced a higher proportion of ROR?t protein than that in TPC-1 cell line without lymph node metastasis. In PTC patients with HT, serum IL-17 concentration was negatively correlated with lymph node metastasis in the central group. CONCLUSIONS:ROR?t may play an anti-tumor role in reducing thyroid cell damage by reducing the thyroid autoimmune antibodies TPOAb and TGAb in the PTC population in Yunnan plateau region.

SUBMITTER: Zeng R

PROVIDER: S-EPMC6220227 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Positive effect of RORγt on the prognosis of thyroid papillary carcinoma patients combined with Hashimoto's thyroiditis.

Zeng Rong R Lyu Yi Y Zhang Guoqiao G Shou Tao T Wang Kai K Niu Heng H Yan Xinmin X

American journal of translational research 20181015 10

<h4>Objectives</h4>To investigate the positive effect of Th17 cells on the prognosis of patients with PTC and HT.<h4>Methods</h4>The expression of nuclear specific marker RORγt of Th17 cells in fresh and paraffin thyroid tissues and serum specimens were analyzed. Flow cytometry was used to detect the formation rates of Th17 cells (CD3<sup>+</sup>CD8<sup>-</sup>IL-17A<sup>+</sup>/CD3<sup>+</sup>CD8<sup>-</sup>%) at different time points after co-culture of thyroid papillary carcinoma cell line (T ...[more]

PMID: 30416647

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Project description:BackgroundHashimoto's thyroiditis (HT) is the most prevalent inflammatory disorder of the thyroid gland. Current studies have reported the coexistence rate between HT and papillary thyroid carcinoma (PTC) is quite high. The objective of this study was to evaluate the impact of HT on the predictive factors of central compartment lymph node metastasis (CLNM) in PTC.MethodsA retrospective investigation was performed on PTC patients. They were subclassified into HT and non-HT groups. The results of preoperative neck ultrasound (US) examinations were reviewed. The clinical characteristics and the predictive value for CLNM were explored and compared between the two groups.ResultsA total of 756 patients were included in this study. There were more female patients (86.1%) in the PTC coexistent with the HT group than non-HT group. The patients with HT group had higher preoperative serum level of TSH. There was statistically significant difference between the HT patients and non-HT patients in nodular vascularization. Univariate and multivariate analyses showed that male, age ≤45 years old, tumor diameter >1 cm, and presence of suspicious central compartment lymph node on US, irregular nodular shape, multifocal carcinoma were independent predictive factors of CLNM in PTC patients. It was showed that male, age ≤45 years old, tumor diameter >1 cm, multifocality, and presence of suspicious central lymph node on US were risk factors for CLNM in non-HT patients. Only tumor diameter >1 cm and presence of suspicious central lymph node on US were independently correlated with CLNM in HT patients. The sensitivity of the multivariate model was 63.5%, and specificity was 88.9% for prediction CLNM in HT patients. For non-HT patients, the AUC was 80.6%, the sensitivity of the multivariate model was 64.5%, and specificity was 85.2.ConclusionPTC combined with HT is more common in women, and TSH level in HT group is higher than that in patients with PTC alone. Regardless of that HT is not a related risk factor of CLNM in PTC, our result suggested that different predictive systems should be used for HT and non-HT patients respectively to have a more accurate evaluation of CLNM in clinic.

Dataset Information

Positive effect of ROR?t on the prognosis of thyroid papillary carcinoma patients combined with Hashimoto's thyroiditis.

Publications

Positive effect of RORγt on the prognosis of thyroid papillary carcinoma patients combined with Hashimoto's thyroiditis.

Similar Datasets

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