US10 Protein Is Crucial but not Indispensable for Duck Enteritis Virus Infection in Vitro.

ABSTRACT: To investigate the function of the duck enteritis virus (DEV) tegument protein US10, we generated US10 deletion and revertant mutants (?US10 and US10FRT) via two-step RED recombination based on an infectious BAC clone of DEV CHv-BAC-G (BAC-G). In multistep growth kinetic analyses, ?US10 showed an approximately 100-fold reduction in viral titer, while the genome copies decreased only 4-fold compared to those of BAC-G. In one-step growth kinetic analyses, there were no significant differences in genome copies among BAC-G, ?US10 and US10FRT, but ?US10 still showed a 5- to 20-fold reduction in viral titer, and the replication defect of ?US10 was partially reversed by infection of US10-expressing cells. The transcription levels of Mx, OASL, IL-4, IL-6 and IL-10 in ?US10-infected duck embryo fibroblasts (DEFs) were significantly upregulated, while TLR3 was downregulated compared with those in BAC-G-infected DEFs. Taken together, these data indicated that US10 is vital for DEV replication and is associated with transcription of some immunity genes.

SUBMITTER: Ma Y 

PROVIDER: S-EPMC6220328 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature