Dataset Information

Increased frequency of systemic pro-inflammatory V?1⁺ ?? T cells in HIV elite controllers correlates with gut viral load.

ABSTRACT: ?? T cells predominate in the intestinal mucosa and help maintain gut homeostasis and mucosal immunity. Although HIV infection significantly alters these cells, what drives these perturbations is unclear. Growing evidence suggests that impaired intestinal immune function in HIV leads to chronic immune activation and disease progression. This occurs even in HIV controllers - individuals with undetectable HIV viremia without antiretroviral therapy (ART). We show that V?1⁺ cells, a subset of ?? T cells described as being important in intestinal barrier function, increase in frequency in HIV-infected individuals, including HIV controllers. These cells resemble terminally differentiated effector memory cells, producing the pro-inflammatory cytokines IFN?, TNF?, and MIP-1? upon stimulation. Importantly, pro-inflammatory V?1⁺ cell frequency correlates with levels of HIV RNA in intestinal tissue but not in plasma. This study supports a model in which local viral replication in the gut in HIV controllers disrupts the phenotype and function of V?1⁺ cells, a cell type involved in the maintenance of epithelial barrier integrity, and may thereby contribute to systemic immune activation and HIV disease progression.

SUBMITTER: Olson GS

PROVIDER: S-EPMC6220338 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Increased frequency of systemic pro-inflammatory Vδ1<sup>+</sup> γδ T cells in HIV elite controllers correlates with gut viral load.

Olson Gregory S GS Moore Sarah W SW Richter James M JM Garber John J JJ Bowman Brittany A BA Rawlings Crystal A CA Flagg Meaghan M Corleis Björn B Kwon Douglas S DS

Scientific reports 20181107 1

γδ T cells predominate in the intestinal mucosa and help maintain gut homeostasis and mucosal immunity. Although HIV infection significantly alters these cells, what drives these perturbations is unclear. Growing evidence suggests that impaired intestinal immune function in HIV leads to chronic immune activation and disease progression. This occurs even in HIV controllers - individuals with undetectable HIV viremia without antiretroviral therapy (ART). We show that Vδ1<sup>+</sup> cells, a subse ...[more]

PMID: 30405182

Similar Datasets

Project description:BackgroundWe previously reported that Vδ2+Vγ9+ γδ T cells were significantly decreased in multiple sclerosis (MS) patients without disease-modifying therapies (untreated MS) and were negatively correlated with Expanded Disability Status Scale (EDSS) scores, suggesting protective roles of Vδ2+Vγ9+ γδ T cells. Interferon-β (IFN-β) is one of the first-line disease-modifying drugs for MS. However, no previous studies have reported changes in γδ T cell subsets under IFN-β treatment. Therefore, we aimed to clarify the effects of the long-term usage of IFN-β on γδ T cell subsets in MS patients.MethodsComprehensive flow cytometric immunophenotyping was performed in 35 untreated MS and 21 MS patients on IFN-β for more than 2 years (IFN-β-treated MS) including eight super-responders fulfilling no evidence of disease activity criteria, and 44 healthy controls (HCs).ResultsThe percentages of Vδ2+Vγ9+ cells in γδ T cells were significantly lower in untreated and IFN-β-treated MS patients than in HCs. By contrast, the percentages of Vδ1-Vδ2-Vγ9- cells in γδ T cells were markedly higher in IFN-β-treated MS patients than in HCs and untreated MS patients (both p < 0.001). A significant negative correlation between the percentages of Vδ2+Vγ9+ cells in γδ T cells and EDSS scores was confirmed in untreated MS but not evident in IFN-β-treated MS. Moreover, class-switched memory B cells were decreased in IFN-β-treated MS compared with HCs (p < 0.001) and untreated MS patients (p = 0.006). Interestingly, the percentages of Vδ1-Vδ2-Vγ9- cells in γδ T cells were negatively correlated with class-switched memory B cell percentages in all MS patients (r = - 0.369, p = 0.005), and the percentages of Vδ1-Vδ2-Vγ9- cells in Vδ1-Vδ2- γδ T cells were negatively correlated with EDSS scores only in IFN-β super-responders (r = - 0.976, p < 0.001).ConclusionsThe present study suggests that long-term usage of IFN-β increases Vδ1-Vδ2-Vγ9- γδ T cells, which are associated with a better outcome, especially in IFN-β super-responders. Thus, increased Vδ1-Vδ2-Vγ9- cells together with decreased class-switched memory B cells may contribute to the suppression of disease activity in MS patients under IFN-β treatment.

Dataset Information

Increased frequency of systemic pro-inflammatory V?1⁺ ?? T cells in HIV elite controllers correlates with gut viral load.

Publications

Increased frequency of systemic pro-inflammatory Vδ1<sup>+</sup> γδ T cells in HIV elite controllers correlates with gut viral load.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Increased frequency of systemic pro-inflammatory V?1+ ?? T cells in HIV elite controllers correlates with gut viral load.

Publications

Increased frequency of systemic pro-inflammatory Vδ1<sup>+</sup> γδ T cells in HIV elite controllers correlates with gut viral load.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Increased frequency of systemic pro-inflammatory V?1⁺ ?? T cells in HIV elite controllers correlates with gut viral load.