Dataset Information

Storage related haematological and biochemical changes in Plasmodium falciparum infected and sickle cell trait donor blood.

ABSTRACT:

Background

In sub-Saharan Africa where sickle cell trait (SCT) and malaria is prevalent, significant proportions of blood donors may be affected by one or more of these abnormalities. The haemato-biochemical properties of SCT and asymptomatic malaria in donor blood have not been evaluated. This study evaluated the haemato-biochemical impact of SCT and asymptomatic malaria infections in citrate-phosphate-dextrose-adenine (CPDA-1) stored donor blood units.

Methods

Fifty-milliliters of sterile CPDA-1 anti-coagulated blood were drained into the sample pouch attached to the main blood bag. Ten units each of sickle cell/malaria negative, sickle cell and malaria positive blood were analyzed. Baseline and weekly haematological profiling and week 1, 3 and 5 concentrations of plasma haemoglobin, % haemolysis, sodium, potassium and chloride and lactate dehydrogenase (LDH) were assayed. Differences between baseline and weekly data were determined using one-way analysis of variance (ANOVA) and Kruskal-Wallis test, whereas differences between baseline parameters and week 1-3 data pairs were determined using paired t-test. P-value ResultsStorage of SCT and malaria infected blood affected all haematological cell lines. In the SCT donors, red blood cells (RBC) (4.75?×?10¹²/L?±?1.43^baseline to 3.49?×?10¹²/L?±?1.09^week-5), haemoglobin (14.45 g/dl?±?1.63^baseline to 11.43 g/dl?±?1.69^week-5) and haematocrit (39.96%?±?3.18^baseline to 33.22%?±?4.12^week-5) were reduced. In the asymptomatic malaria group, reductions were observed in RBC (5.00?×?10¹²/L?±?0.75^baseline to 3.72?×?10¹²/L?±?0.71^week-5), haemoglobin (14.73 g/dl?±?1.67^baseline to 11.53 g/dl?±?1.62^week-5), haematocrit (42.72%?±?5.16^baseline to 33.38%?±?5.80^week-5), mean cell haemoglobin concentration (35.48 g/dl?±?1.84^baseline to 35.01 g/dl?±?0.64^week-5) and red cell distribution width coefficient of variation (14.81%?±?1.54^baseline to 16.26%?±?1.37^week-5). Biochemically, whereas plasma LDH levels significantly increased in asymptomatic malaria blood donors (319% increase at week 5 compared to baseline), SCT blood donors had the most significant increase in plasma potassium levels at week 5 (382% increase). Sodium ions significantly reduced in SCT/malaria negative and sickle cell trait blood at an average rate of 0.21 mmol/L per day. Moreover, elevations in lymphocytes-to-eosinophils and lymphocytes-to-neutrophils ratios were associated with SCT and malaria positive blood whilst elevation lymphocytes-to-basophils ratio was exclusive to malaria positive blood.

Conclusion

Severe storage lesions were significant in SCT or malaria positive donor blood units. Proper clinical evaluation must be done in prospective blood donors to ensure deferral of such donors.

SUBMITTER: Aninagyei E

PROVIDER: S-EPMC6220467 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Storage related haematological and biochemical changes in <i>Plasmodium falciparum</i> infected and sickle cell trait donor blood.

Aninagyei Enoch E Doku Emmanuel Tetteh ET Adu Patrick P Egyir-Yawson Alexander A Acheampong Desmond Omane DO

BMC hematology 20181106

<h4>Background</h4>In sub-Saharan Africa where sickle cell trait (SCT) and malaria is prevalent, significant proportions of blood donors may be affected by one or more of these abnormalities. The haemato-biochemical properties of SCT and asymptomatic malaria in donor blood have not been evaluated. This study evaluated the haemato-biochemical impact of SCT and asymptomatic malaria infections in citrate-phosphate-dextrose-adenine (CPDA-1) stored donor blood units.<h4>Methods</h4>Fifty-milliliters ...[more]

PMID: 30450212

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