Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose-regulated GTH1 promoter of Pichia pastoris.

ABSTRACT: Protein production in Pichia pastoris is often based on the methanol-inducible P _AOX1 promoter which drives the expression of the target gene. The use of methanol has major drawbacks, so there is a demand for alternative promoters with good induction properties such as the glucose-regulated P _GTH1 promoter which we reported recently. To further increase its potential, we investigated its regulation in more details by the screening of promoter variants harboring deletions and mutations. Thereby we could identify the main regulatory region and important putative transcription factor binding sites of P _GTH1 . Concluding from that, yeast metabolic regulators, monomeric Gal4-class motifs, carbon source-responsive elements, and yeast GC-box proteins likely contri

SUBMITTER: Prielhofer R 

PROVIDER: S-EPMC6221138 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature