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Hepatocyte-like cells generated by direct reprogramming from murine somatic cells can repopulate decellularized livers.


ABSTRACT: Direct reprogramming represents an easy technique to generate induced hepatocytes (iHeps) from somatic cells. However, current protocols are accompanied by several drawbacks as iHeps are heterogenous and lack fully mature phenotypes of primary hepatocytes. Here, we established a polycistronic expression system to induce the direct reprogramming of mouse embryonic fibroblasts towards hepatocytes. The resulting iHeps are homogenous and display key properties of primary hepatocytes, such as expression of hepatocyte markers, albumin secretion, and presence of liver transaminases. iHeps also possess the capacity to repopulate decellularized liver tissue and exhibit enhanced hepatic maturation. As such, we present a novel strategy to generate homogenous and functional iHeps for applications in tissue engineering and cell therapy.

SUBMITTER: Chen C 

PROVIDER: S-EPMC6221165 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Hepatocyte-like cells generated by direct reprogramming from murine somatic cells can repopulate decellularized livers.

Chen Chen C   Pla-Palacín Iris I   Baptista Pedro M PM   Shang Peng P   Oosterhoff Loes A LA   van Wolferen Monique E ME   Penning Louis C LC   Geijsen Niels N   Spee Bart B  

Biotechnology and bioengineering 20180917 11


Direct reprogramming represents an easy technique to generate induced hepatocytes (iHeps) from somatic cells. However, current protocols are accompanied by several drawbacks as iHeps are heterogenous and lack fully mature phenotypes of primary hepatocytes. Here, we established a polycistronic expression system to induce the direct reprogramming of mouse embryonic fibroblasts towards hepatocytes. The resulting iHeps are homogenous and display key properties of primary hepatocytes, such as express  ...[more]

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