Ontology highlight
ABSTRACT:
SUBMITTER: Okomoda VT
PROVIDER: S-EPMC6223236 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Okomoda Victor T VT Koh Ivan C C ICC Hassan Anuar A Amornsakun Thumronk T Moh Julia H Z JHZ Shahreza Sheriff Md SM
PeerJ 20181105
To obtain well spread chromosomes, the cytogenetic protocol for <i>Pangasianodon hypophthalmus</i> and <i>Clarias gariepinus</i> were optimized. This includes, the colchicine concentration (0.01%, 0.025%, 0.05%)/exposure duration (1, 3, and 5 h), hypotonic solution (distilled water or 0.075M KCl solution)/exposure duration (30 min, 1, and 2 h), the time of cell suspension preparation (at hypotonic treatment or before slide preparation) and chromosome aging period (0, 3, and 7 days in Carnoy's fi ...[more]